草菇VH3菌株及其单孢后代间的差异分析

摘要

从菌落形态、菌丝生长速度及RAPD分析等方面，研究草菇菌株VH3及其14个单孢菌株间的生长与遗传差异。研究结果显示，这些供试菌株的菌落形态、菌丝长势、菌丝生长速度存在较大差异；通过RAPD指纹分析，发现VH3及其单孢菌株的遗传相似系数在0.50~0.96之间，在基因水平上供试菌株间的遗传差异较大。草菇菌株VH3及其单孢后代的表型与基因型均存在显著性差异，表明VH3菌株借助有性生殖过程获得了丰富的生物学变异。%The colony morphology, the mycelial extension growth rate and the genetic diversity of strain VH3(Volvariellavolvacea) and its 14 single spore isolates (SSIs) based on RAPD analysis were studied. Results showed that the colony morphology, the myceli-um growth vigor and the mycelial extension growth rate were significantly different. The genetic similarity coefficient of VH3 and its SSIs was from 0. 50 to 0. 96 by RAPD analysis. The significant differences of phenotype and genotype in strain VH3 and its SSIs in-dicated that VH3 got abundant biomutations by sexual reproduction.