JNK1基因在金鱼性腺不同发育时期的表达分析

摘要

The objective of this study was to explore the correlation of JNK1 gene in the process of gonad development of Goldfish (Carassius auratus).The expression of JNK1 was studied and analyzed in different developmental phases of gonad by using quantitative real-time PCR and Western blotting.It was found that gonadosomatic index (GSI) of males and females showed significant seasonal variation with the development process of gonad and was the maximium during the breeding period (P <0.05).The qPCR analysis revealed that the expression of JNK1 firstly increased and then decreased at the development phases of ovary, reached the peak level at maturity phase (P <0.05),4.0 times higher before sexual maturity, 1.4 times higher degradation phase and 2.1 times higher at the rest phase compared with that at maturity phase.In the testis, the expression of JNK1 was found to be trace enhanced compared with before sexual maturity phase, however, the expression changes had no significant difference in the whole development process.Western blotting analysis showed that expression tendency of JNK1 protein was in line with that of JNK1 mRNA.Together, these results suggest that JNK1 plays an important role in the processes of the development of ovary and promoting the maturation of oocytes in fish.%为探明JNK1(c-Jun N-terminal kinase 1)在鱼类性腺发育过程中的相关性,以模式动物金鱼为实验对象,采用实时荧光定量PCR(qPCR)和蛋白免疫印迹(Western blotting)技术检测JNK1基因及其蛋白在金鱼精巢和卵巢不同发育时期的表达水平.结果表明:金鱼雄鱼和雌鱼的性腺指数(GSI)显示性腺在发育过程中呈明显的季节变化规律,其中在性腺成熟期GSI最高(P<0.05).qPCR检测结果显示JNK1在卵巢发育时期的表达量表现为先升高后降低的趋势,其在卵巢成熟期的表达量最高(P<0.05),为成熟前期、退化吸收期和修整恢复期表达量的4.0、1.4和2.1倍;JNK1在精巢不同发育时期的表达量总体呈现出微量变化的趋势;在精巢成熟前期表达最低,成熟期、退化吸收期和修整恢复期表达量较成熟期有微量升高,且精巢发育全过程中的表达变化均无显著性差异.Western blotting检测结果显示其蛋白水平表达模式与mRNA的表达水平吻合.研究表明,JNK1在一定程度上与鱼类卵巢发育相关,可能在促进卵细胞的成熟方面具有重要作用.