首页> 外文会议>International Conference on Biotechnology and Bioengineering >Cloning and expression analysis of FaPR-1 gene in strawberry
【24h】

Cloning and expression analysis of FaPR-1 gene in strawberry

机译:FAPR-1基因在草莓中的克隆与表达分析

获取原文

摘要

The FaPR-1 gene was cloned by RT-PCR from 'Benihoppe' strawberry and its bioinformatics analysis was conducted. The results showed that the open reading frame was 483 bp encoding encoding l60 amino acids which protein molecular weight and theoretical isoelectricity were 17854.17 and 8.72 respectively. Subcellular localization prediction shows that this gene is located extracellularly. By comparing strawberry FaPR-l and other plant Pathogenesis-related protein, homology and phylogenetic tree construction showed that the homology with grapes, peach is relatively close. In the treatments of ABA, sucrose and the mixture of the two, the expression of FaPR-1 in strawberry fruit were significantly increased.
机译:通过RT-PCR克隆FAPR-1基因,从'圆波普普'草莓克隆,并进行其生物信息学分析。结果表明,开放阅读帧为483bp编码,编码L60氨基酸,蛋白质分子量和理论等电性分别为17854.17和8.72。亚细胞定位预测表明该基因位于细胞外。通过比较草莓FAPR-L和其他植物发病机制相关的蛋白质,同源性和系统发育树施工表明,葡萄的同源性,桃相对较近。在ABA,蔗糖和两者混合物的处理中,FAPR-1在草莓果实中的表达显着增加。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号