斑马鱼CYP11 a1基因在不同性腺发育时期的表达

摘要

The expression of CYP11 a1 gene was studied and analyzed in four tissues and three development phases in gonad of zebrafish Danio rerio by quantitative Real-time PCR (qRT-PCR). It was found that the CYP11a1 gene was expressed in ovary, testis, muscle and brain, the minimum in brain(P<0. 05), 204. 7 times higher in ovary, 38. 9 times higher in testis, and 17. 0 times higher in muscle compared with that in brain, indicating that the CYP11a1 gene showed a certain extent tissue difference in expression. The qRT-PCR analysis revealed that the ex-pression of CYP11 a1 gene was found to be decreased first in the early development phases of ovary and then en-hanced in the later development phases of ovary, the minimum at maturity phase(P>0. 05), 1. 7 times higher be-fore sexual maturity and 1. 5 times higher after sexual maturity compared with that at maturity phase. In the testis, however, the expression of CYP11a1 gene was found to be enhanced first in the early development phases of testis and then decreased in the later development phases of testis, the maximum at the sexual maturity(P<0. 05), 6. 5 times higher before sexual maturity and 13. 4 times higher than that after the sexual maturity. The findings indicated that the expression of CYP11a1 gene was involved in gonad development and spermatogenesis in zebrafish, though the expression pattern of CYP11a1 gene was different in the ovary and the testis.%采用实时荧光定量PCR技术，分析了斑马鱼Danio rerio的CYP11a1基因在成鱼4个组织和性腺发育3个时期的表达情况。结果表明： CYP11a1基因在卵巢、精巢、肌肉和脑组织中均有表达且存在差异，在脑中表达量最低(P<0.05)，在卵巢、精巢、肌肉中的表达量分别为脑中表达量的204.7倍、38.9倍、17.0倍； CYP11a1基因在3个卵巢发育时期的表达量表现为先降低后升高的趋势，其在卵巢成熟期的表达量最低(P>0.05)，在卵巢成熟前期、卵巢成熟后期的表达量分别为卵巢成熟期表达量的1.7倍、1.5倍；CYP11a1基因在3个精巢发育时期的表达量表现为先升高后降低的趋势，在精巢成熟期表达量最高( P<0.05)，分别为精巢成熟前期、精巢成熟后期表达量的6.5倍、13.4倍。研究表明，尽管CYP11a1基因在卵巢和精巢发育过程中的表达规律不同，但CYP11a1基因在一定程度上与斑马鱼的性腺发育相关，其可能在促进斑马鱼精子成熟中发挥一定作用。