Objective: To improve bone formation by mineralizing silk fibroin scaffold with calcium phosphate. Methods: Silk fibroin was immerged into calcium phosphate solution, and then mineralized on its surface using hydrothermal method. The characteristics of silk fibroin surface-modified were measured by scanning electron microscopy ( SEM ) , energy dispersive X-ray microanalysis, X-ray diffraction and Fourier transform infrared spectroscopy. Results: SEM indicated that the surface of silk fibroin was mostly coated by calcium phosphate. The patterns of silk fibroin surface-modified were not changed after immerging in solution, and carbonate apatite distributed on its surface. Conclusions: As a natural material, the silk fibroin possesses good biocompatibility. Silk fibroin/ CaP composite as scaffold material is a promising biomaterial.%目的:通过对蚕丝表面进行磷酸钙涂层,从而增加其成骨效果.方法:将脱胶后的蚕丝放入磷酸钙溶液中,用水热法对蚕丝表面进行矿化,运用扫描电镜、能谱分析仪、X线衍射仪、傅立叶红外对表面改良的蚕丝进行理化性能检测.结果:磷酸钙表面改良处理后磷酸钙均匀涂布于蚕丝表面,矿化蚕丝初始形态并未发生明显改变,表面存在磷酸灰石成分.结论:蚕丝作为一种天然生物纤维,具有良好的生物相容性.经磷酸钙涂层的蚕丝,作为组织工程支架材料具有广阔的发展前景.
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