荧光定量RT-PCR技术检测健康人外周血γδT细胞细胞受体分子Vδ1~8亚群分布

摘要

目的：：检测和分析健康成人外周血中γδT细胞的T细胞受体( TCR)分子Vδ1~8亚群分布情况。方法：采集16名健康成人外周血，荧光定量反转录聚合酶链式反应检测其γδT细胞TCR分子Vδ1~8亚群的基因相对表达量，计算各亚群所占百分比。结果：健康成人外周血γδT细胞TCR分子Vδ1~8亚群的比例分别为：Vδ1(7.19±0.33)%、Vδ2(54.27±7.9)%、Vδ3(20.23±0.03)%、Vδ4(3.80±0.02)%、Vδ5(2.08±0.02)%、Vδ6(0.81±0.25)%、Vδ7(7.21±0.02)%、Vδ8(4.41±0.04)%。 Vδ2亚群均明显高于其他亚群(PVδ3>Vδ1>Vδ8>Vδ7>Vδ4>Vδ5>Vδ6。%Objective:To observe the distribution of Vδ1 to 8 subsets of the T cell receptor(TCR) molecule in the γδT cell of the human peripheral blood using real-time PCR. Methods:The mRNA expressions of the Vδ1 to 8 subsets of TCR molecule in theγδT cell in 16 healthy people peripheral blood were detected by real-time PCR,the respective percentage of whose were calculated. Results:The ratio of Vδ1,2,3,4,5,6,7 and 8 subsets were(7. 19 ± 0. 33)%,(54. 27 ± 7. 9)%,(20. 23 ± 0. 03)%,(3. 80 ± 0. 02)%,(2. 08 ± 0. 02)%,(0. 81 ± 0. 25)%,(7. 21 ± 0. 02)% and(4. 41 ± 0. 04)%,respectively. The ratio of Vδ2 was significantly higher than that in other subsets(PVδ3>Vδ1>δV8>Vδ7>Vδ4>Vδ5>Vδ6.