小剂量地塞米松对脓毒症小鼠CD11 b+ Gr-1+髓源抑制性细胞的影响

摘要

Objective:To investigate the effects of low dose of dexamethasone on CD11b+ Gr-1 + myeloid-derived suppressor cells ( MDSCs) in septic mice induced by lipopolysaccharide( LPS) . Methods:The septic mice model was established by the intraperitoneal injection of LPS in BALB/c mice. The mice were randomly divided into the normal control group( NC group) ,sepsis group( SE group) and sepsis combined with dexamethasone group(SD group). The levels of serum interferon-γ and interleukin-10 were detected at 6,12 and 24 hours. The percentage of CD11b+ Gr-1 + MDSCs in spleen and bone marrow of mice were detected at 12,24,48 and 72 hours. Results:Compared with the NC group,the levels of interferon-γ at 6 hours and interleukin-10 at 12 hours in SE group and SD group increased significantly(P <0. 05 to P <0. 01),and the difference of which between SE group and SD group was not statistically different( P<0. 05). Compared with the NC group,the percentages of MDSCs in spleen at 12 to 72 hours in SE group and at 12 to 48 hours in SD group increased significantly( P<0. 01),and the differences of the percentage of MDSCs at 24 to 72 hours between SE group and SD group were statistically different( P<0. 05). Compared with the NC group,the percentages of MDSCs in bone marrow of SE and SD group increased significantly at 48 hours and 72 hours( P<0. 05 to P<0. 01). the differences of which at 48 and 72 hours between SE and SD groups were statistically significant(P<0. 05 and P<0. 01). Conclusions:Low dose of dexamethasone can inhibit the production and accumulation of MDSCs in septic mice induced by LPS.%目的：：观察小剂量地塞米松对脂多糖(LPS)诱导的脓毒症小鼠CD11b+Gr-1+髓源抑制性细胞(MDSCs)的影响。方法：将145只BALB/c雄性小鼠随机分成正常对照组(NC组)35只,脓毒症组(SE组)和小剂量地塞米松干预组(SD组)各55只；SE组和SD组腹腔注射LPS 10 mg/kg制备脓毒症小鼠模型,NC组腹腔注射等量0.9%氯化钠注射液。 SD组于注射LPS 2 h后经尾静脉注入地塞米松0.3 mg/kg,SE组和NC组经尾静脉注入等量0.9%氯化钠注射液。注射LPS后时刻记为0 h,于6、12、24 h检测血清干扰素-γ及白细胞介素-10水平,于12、24、48、72 h检测小鼠脾脏及骨髓中CD11b+Gr-1+ MDSCs的百分比。结果：与NC组比较,SD组及SE组在实验6 h干扰素-γ和12 h白细胞介素-10水平均增加(P<0.05~P<0.01)；SD组与SE组差异亦有统计学意义(P<0.05)。与NC组比较,SE组脾脏MDSCs百分比在12~72 h均明显升高(P<0.01),SD组在12~48 h亦均升高(P<0.01)；而SE组和SD组在24~72 h差异亦均有统计学意义(P<0.01)。与NC组比较,SE组和SD组骨髓MDSCs百分比在48 h和72 h均升高(P<0.05~P<0.01)；而SE组和SD组在48 h和72 h差异亦均有统计学意义(P<0.05和P<0.01)。结论：小剂量地塞米松可抑制LPS诱导的脓毒症小鼠MDSCs的产生及聚集。