超声刺激对海藻酸钠微球机械特性和释放特性的影响

摘要

The aim of this research is to fabricate alginate beads and measure their mechanical properties; to investigate the changes of mechanical properties and release properties of alginate beads under ultrasonic stimulation. Alginate bead was fabricated with the extrusion method. Centered on bead under stable condition, its shear module was analyzed with the compression test technique and Hertz theory. Alginate bead was sonicated by different ultrasonic stimulations. By measuring the shear module of beads sonicated, effects of ultrasonic stimulation on the mechanical property of alginate beads incorporating bovine serum albumin ( BSA ) were investigated, and its passive release property was analyzed by Higuchi model. The results show that the shear module of alginate beads is 42.5 kPa, the ultrasonic stimulation has no effect on their mechanical properties, and their release rate can be changed by ultrasonic stimulation power and stimulation time.%为了测量海藻酸钠微球的剪切模量,并检测在超声刺激下海藻酸钠微球的机械特性和释放特性的变化,采用传统挤压方法制备海藻酸钠微球.在稳定状态下,通过压缩实验技术和Hertz理论模型分析得到海藻酸钠微球的剪切模量.采用不同的超声刺激海藻酸钠微球,通过测量其剪切模量,观察超声刺激对微球的机械特性的影响.实验对于包埋牛血清蛋白(bovine serum albumin,BSA)的海藻酸钠微球,采用Higuchi模型分析其释放特性.结果表明：海藻酸钠微球的剪切模量为42.5 kPa,超声刺激对微球的剪切模量无明显影响,而其释放速率受到超声刺激时间和功率的影响.