对拮抗放线菌菌株F1发酵液的抑菌谱及稳定性做了研究.抑菌活性实验表明:菌株F1发酵液对供试17种植物病原真菌和7种病原细菌都有较强的抑制活性,在供试的植物病原真菌中,对番茄灰霉病菌和李干腐病菌的抑制作用最强,抑菌圈直径分别为41.04 mm和40.12 mm；病原细菌中,对丁香假单胞菌流泪致病变种的抑制作用最强,抑菌圈直径可达22.15 mm；稳定性实验结果表明:菌株F1发酵液对紫外线、热和酸碱较稳定,在pH为6～10条件下,均能保持较强的抑菌活性,但不耐强酸、强碱；当温度低于60℃时,处理30 min和60min,抑菌活性几乎没变化,仍能保持较强的抑菌效果,但高于80℃时,抑菌活性明显降低.表明该菌株发酵液具有广谱抑菌活性和较强的稳定性,具有一定开发价值.%The inhibitory spectrum and stability of fermentation broths of actinomycete strain Fl were studied. The result of antimicrobial activity experiment showed: the fermentation broths had significant inhibitory effect on 17 tested species of plant pathogenic fungi and 7 tested species of bacteria. The fermentation broths showed the strongest antimicrobial effect against Botrytiscinerea and Polvporus hirsutus among 17 fungi species. The inhibitory diameters of Botrytiscinerea or Polvporus hir-sutus were 41. 04mm and 40. 12 mm, respectively. The antimicrobial diameter of Pseudomonas syringae pv. Lachrymans was the biggest (22. 15mm) among 7 tested bacteria species. The stability of fermentation broths was stable to UV, acid, alkali and heat. The fermentation broths remained high antimicrobial effect at pH6～10, but unstable to strong acid and alkali. There was no obvious change on the antimicrobial effect of fermentation broths from 20℃ to 60℃ for 30min and 60min, but the inhibitory activity of fermentation broths decreased considerably at 80℃ or above.
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