Objective To study the effect of polysaccharides from Flammulina velutipes ( FVP ) on the antioxidant capacity of spleen in aging mice, and to investigate its anti-aging mechanism. Method Sixty ICR mice with half males and half females were randomly divided into six groups according to their body weights, including blank control group,model group,piracetam group,FVP low,medium and high dose group. The mice were raised for six weeks,and were sacrificed at the end of the experiment. The spleens were obtained and the spleen indexes were calculated. The antioxidant indexes including MDA, SOD and GSH-Px in spleen were determined. Results Compared to the model group, spleen indexes and MDA contents in FVP groups significantly decreased (P<0. 05),and the activities of GSH-Px and SOD significantly increased (P<0. 05). Conclusion FVP can induce the antioxidant effect of spleen in aging mice,which may be one of the anti-aging mechanism of FVP .%目的：探讨金针菇多糖对衰老小鼠脾脏抗氧化能力的影响及金针菇多糖的抗衰老机制.方法 ICR小鼠60只,雌雄各半,按体质量随机分为6组：空白对照组,模型组,脑复康组,金针菇多糖( FVP )低、中、高剂量组.实验动物喂饲6周,处死小鼠,取脾组织,计算小鼠脾脏指数；测量脾脏抗氧化指标MDA,SOD,GSH-Px.结果与模型组比较,金针菇多糖组脾脏指数显著下降(P<0.05),MDA含量显著下降(P<0.05),GSH-Px和SOD活性显著上升(P<0.05).结论金针菇多糖可使衰老小鼠脾脏产生抗氧化作用,可能是金针菇多糖抗衰老的作用机制之一.
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