基于响应面法对枯草芽孢杆菌WB600-eg2发酵产内切葡聚糖苷酶条件的优化

摘要

出发菌株枯草芽孢杆菌WB600-eg2是通过分子生物学手段构建的重组菌,内切葡聚糖苷酶酶活为1.174IU/mL.本文通过响应面法对装液量、初始pH、接种量、转速、酵母粉添加量等发酵条件进行优化,得到最优发酵条件为装液量55mL/250mL、初始pH7.2、接种量10%、转速170r/min、酵母粉添加量0.4%.在此发酵条件下测得内切葡聚糖苷酶酶活为1.573IU/mL,比未优化条件下提高了33.9%.%The recombinant strain of Bacillus subtilis WB600-eg2 was constructed by molecular biology methodology.The activity of endoglucanase was 1.174IU/mL.Response Surface Methodology was used to optimize fermentation conditions including packing volume,initial pH,inoculum concentration,rotational speed of the cradle and yeast extract in this study.The optimum fermentation conditions were：packing volume 55 mL/250 mL,initial pH 7.2,inoculum concentration 10%,rotational speed of the cradle 170 r/min and yeast extract 0.4%.The endoglucanase yield was promoted by 33.9% from 1.174IU/mL to 1.573 IU/mL under optimum conditions.