[目的] 建立能够准确测定凤仙花中总黄酮含量的方法.[方法] 分别以芦丁、山柰酚为对照品,可见分光光度法是采用AlCl_3显色,在412 nm波长处测定总黄酮的含量;紫外分光光度法在270 nm处测定总黄酮的含量.[结果] 以芦丁、山柰酚为对照品采用可见分光光度法测得总黄酮含量的平均回收率分别为98.52%、99.72%,RSD分别为2.38%、3.14%;紫外分光光度法测得总黄酮含量的平均回收率分别为97.53%、98.75%,RSD分别为2.71%、2.86%.[结论] 以山柰酚为对照品、采用AlCl3为显色剂的可见分光光度法是一种较理想的测定凤仙花中总黄酮含量的方法.%[Objective] The research aimed to study the determination method of total flavonoids content in Impatiens balsamina L. by visible light and ultraviolet spectrophotometry. [Method] With rutin and kaempferol as reference substances respectively, by using visible light spectrophotometry and AlCl_3 as chromogenic reagent, the content of total flavonoids was determined at the wavelength of 412 nm. By using ultraviolet spectrophotometry, the content of total flavonoids was determined at the wavelength of 270 nm. [Result] With rutin and kaempferol as reference substances respectively, the average recoveries by visible light method were 98.52% and 99.72% ,with RSD of 2.38% and 3.14% resp. The average recoveries by visible light method were 97.53% and 98.75%, with RSD of 2.71% and 2.86% resp. [Coclution] By using visible light spectrophotometry, with kaempferol as reference substance is a kind of more ideal determination method of total flavonoids in Impatiens balsamina L..
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