[Objective] The experimental basis of the development of new type of immune agent was provided through the study on the activity of immune regulator of scrocaffeside A(SA). [Method] The monomer: E-caffeoyl-6-(4-O-β-D-glucopyranosyl)-E-caffeoyl-O-β-D-pyrazole furans glucopyranoside isolated from the roots of Picrorhiza scrophulariiflora , was named as scrocaffeside A(SA) after its identification. The cytokine in the cell supernatants of the mouse, stimulatively in vitro treated by the different concentrations of SA coopered with Con A ion was tested with the method of double-antibody ELISA. [Results] The cytokines of IL-2, IL-12 and IFN-γ in Th1 cells were significantly increased in the treatment of 25 mg/L and 125 mg/L, which reaction between the different concentrations of SA coopered with Con A(5mg/L) and cells lasted 24 and 48 hours and the cytokines of IL-4 and IL-10 in Th2 cell was also significantly increased, only IL-10 was declined in the treatment condition of the reaction time, 48 hours and the concentration of SA, 125 mg/L. [Conclusion] SA could strengthen the expression of cytokines associated with stronger immune function.%[目的]研究Scrocaffeside A(SA)免疫调节活性,为新型免疫增强剂的开发提供试验依据.[方法]从西藏胡黄连的根茎中分离鉴定得到的单体E-咖啡酰基-6-(4-O-β-D-吡喃葡萄糖基)-E-咖啡酰基-O-β-D-吡喃葡萄糖苷,命名为Scrocaffeside A(SA).通过用不同浓度的SA协同Con A体外刺激小鼠脾淋巴细胞, 采用双抗夹心ELISA法,检测细胞上清中细胞因子的分泌量.[结果]不同浓度SA协同Con A(5 mg/L)与细胞作用24 及48 h后,在25和125 mg/L浓度组显著上调了Th1 细胞分泌的主要细胞因子IL-2、IL-12和IFN-γ的分泌量;Th2 细胞分泌的主要细胞因子IL-4和IL-10分泌量也显著升高,仅IL-10的分泌量在48 h,SA浓度为125 mg/L剂量时有所下降.[结论]SA能上调相关细胞因子的表达,具有免疫增强作用.
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