高效液相色谱-四通道紫外可见检测法测定五加皮中原儿茶酸含量

摘要

[目的]建立五加皮中原儿茶酸的含量测定方法.[方法]采用高效液相色谱-四通道紫外可见检测法,以ODSC18(柱长250mm,内径4.6mm,涂布厚度5μm)为色谱柱,流动相为甲醇∶磷酸二氢钠缓冲溶液(浓度0.1 mol/L)=15:85,pH =2.5,测定五加皮中原儿荼酸的含量.四通道紫外检测的定性检测波长分别为245、260、275、290 nm;定量检测波长为260nm.[结果]原儿茶酸在0.5 ～6.0 mg/L范围内线性关系良好(r =0.999 9),平均回收率为99.87％,RSD为2.11％.[结论]该方法测定结果可靠,稳定易行,重复性好,可用于五加皮中原儿茶酸的含量测定.%[Objective]To established a quantitative method of determination of protocatechuic acid in cortex acanthopanacis radicis. [Method] High proficiency liquid chromatography ( HPLC ) coupled with four-channel UV-visible detector was used, C,, chromatographic column, mobile phase was the buffer solution of methanol and sodium dihydrogen phosphate (0.1 mol/L) ,the volume ratio of methanol and sodium dihydrogen phosphate was 15=85 ,pH = 2.5. Qualitative evaluation was done by retention time and four-channel UV spectrum at the same time,and the detec tion wavelength were 245 nm,260 nm,275 nm,290 nm. Selected the best sensitive wave of 260 nm to make quantitative evaluation. Result:The linearity of protocatechuic acid was in the range of 0. 5 -6.0 ml/L (r =0.999 9) ,the average recovery was 99.87% ,the RSD was 2.11%. [Con clusion] The method is reliable,and can be used for quality control of cortex acanthopanacis radicis.