[ Objective ] The research aimed to screen antagonistic endophytic actinomycetes from Medicago sativa. [ Method ] Growth promotion and disease control effect determination has been done by using Mx1, Mx3 and Mx5, which wcre isolated from the healthy Medicago sativa[ Result ] The result that the comparative control of Mx3 extracts could be 56.56％, 44.67％ to Botrytis cinerea and Gaeumannomyces graminis var.tritici, respectively; the control effect of Mx3 extract was higher than it' s suspension. Mx3 extract had good effect on plant height and leaf quantity of tomato growing in the greenhouse; its growth-promoting abihty on wheat and cucumber was higher than water control. [ Conclusion ] Mx3 could promote growth of root, bud on both tomato and wheat, but it could restrain the growth of cucumber root.%[目的]从首蓿内生放线菌中筛选拮抗菌株.[方法]采用已从健康首蓿组织中分离得到的内生放线菌Mx1、Mx3、Mx5进行温室促生防病测定.[结果]菌株Mx3的发酵液对番茄灰霉病的防效可达56.56%,对小麦全蚀病的防效为44.67%;发酵液对2种病害的防效都明显高于菌悬液.对温室番茄株高、叶片数生长影响最明显;对小麦、黄瓜的促生均高于对照.[结论]菌株Mx3促生效果最好,其发酵液的防病效果明显优于菌悬液.
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