[Objective] To explore the feasibility of biosynthesizing small caliber bacterial cellulose tube by using the batch enclosure method to culture Acetobacter xylinum, and characterize the tube. [ Method] I1 sing coconut water as medium and silicone tube as the vector of oxygen permeation, bacterial cellulose tube was cultured in closed vessel. Then the water content and porosity of cultured products were determined, the effects of thermal drying and freeze drying on cultured products were compared, and the cultured products were scanned. [ Result] Small caliber bacterial cellulose tube can be synthesized by using batch closed culture method. Compared thermal drying with freeze drying, the latter was more suitable to tube storage and next research, compact mesh structure of tube wall and layered structure of tube section were observed by using SEM to scan tube wall and cross section. [Conclusion] Small caliber bacterial cellulose tube can be synthesized by using batch closed culture method, and tube wall with nanometer aperture had potential to be used as separation membrane.%[目的]采用间歇式密闭培养法培养木醋杆菌,探讨该方法合成小口径细菌纤维素管的可行性并对管进行表征研究.[方法]以椰子水为培养基、硅胶管为渗氧载体,在密闭罐中培养木醋杆菌,以合成小口径细菌纤维素管,之后测定产物的湿态含水率,孔隙率,比较热干燥与冷冻干燥2种干燥条件的差异,并通过扫描电镜(SEM)观察培养产物的形貌.[结果]密闭式间歇培养法能生物合成小口径细菌纤维素管；通过不同干燥条件的比较发现,相比于热干燥,冷冻干燥更适合于管的储存及后续工作；通过扫描电镜观察合成的管壁及横截面结构,发现该管壁处有较致密的网孔结构,且管断面有明显的层状结构.[结论]小口径细菌纤维素管可通过间歇式密闭法合成,且管的管壁有纳米级孔径,提示其有作为分离膜的潜力.
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