敖汉细毛羊Ⅰ型内根鞘角蛋白基因表达模式的研究分析

摘要

[目的]探讨细毛羊毛囊生长期及休止期特异基因的表达模式以及该模式对敖汉细毛羊选育的意义.[方法]通过基因芯片技术对敖汉细毛羊羊毛生长期和休止期的颈部、腹股沟部的上皮基因表达进行检测.[结果]统计分析Ⅰ型内根鞘角蛋白基因KRT25、KRT26、KRT27和KRT28在细毛羊颈部与腹股沟部的表达,发现该基因在细毛羊生长期颈部表达量极显著高于腹股沟部的表达量(差异倍数＞2,P＜0.01)；对比分析生长期与休止期该基因的表达变化,结果显示在腹股沟部所有芯片涵盖的Ⅰ型内根鞘角蛋白基因的表达都表现出由生长期进入休止期的显著下调(P＜0.05),除KRT25(LOC443079)外,其他基因的表达变化都在2倍以上.[结论]该研究结果表明Ⅰ型内根鞘角蛋白基因家族的表达与特异部位羊毛密度的控制以及整个毛囊发育生长周期都有着重要的联系.%[Objective] The paper was to study the expression pattern of specific genes during wool anagen and telogen and it significance to Aohan wool sheep breeding, [ Method ] Mieroarrays were used to detect the expression of different genes between neck and groin during stages of anagen and telogen in Aohan wool sheep. [Result] The expression of type I IRS keratin genes KRT15, KRT26, KRT27 and KRT28 in neck and groin of wool sheep were statistically analyzed, and the result showed that the expression in neck during anagen stage was significantly higher than that in groin( The multiple is more than 2 times, P < 0. 01). The expression changes of the gene during stages of anagen and telogen were compared, and the results showed that the expression of all Type I IRS Keratin genes performed in groin showed significant decrease from anagen stage to telogen stage ( P < 0. 05 ) , the expression changes of other gene were two times higher, except KRT25 (LDC443079). [Conclusion] The result indicated that the expression of type [ IRS Keratin genes had important link with control of wool density in specific part and the whole wool development cycle.