转化生长因子βⅠ型受体基因在敖汉细毛羊不同组织及发情时期卵巢中的表达分析

摘要

This study was aimed to investigate the expression of transforming growth factor-beta receptor Ⅰ(TGF-βR Ⅰ) in main tissue of AoHan Fine-wool sheep,the expression variations of ovary in different estrous states,and the mechanism of TGF-βRⅠ gene regulation in seasonal estrus of AoHan Fine-wool sheep.Twenty-four seasonal estrus AoHan Fine-wool sheep were chosen and divided into 4 groups of anestrus, proestrus, estrus and dioestrum with 6 sheep per group.First of all,the expression of TGF-βRⅠ gene in twelve tissues (thyroid,hypothalamus,hypophysis,ovary,adrenal gland,heart,liver,spleen,lung,kidney,pancreas and muscle) of AoHan Fine-wool sheep in the estrous stage and its expression variations in ovary of anestrus,proestrus,estrus and dioestrum were detected by Real-time PCR.The results showed that TGF-βRⅠ gene expressed in all the 12 tissues,and its expression in ovary and thyroid were extremely significantly higher than that in others (P<0.01).The expression of TGF-βRⅠ gene in hypothalamus,hypophysis,pancreas,adrenal gland, spleen and lung were high,which were significant higher than heart,liver,kidney and muscle (P<0.05).The expression levels of TGF-βRⅠgene in proestrus were extremely higher than other stages (P<0.01).The expression in dioestrum were the lowest which extremely lower than other stages (P<0.01).The above results indicated that TGF-βRⅠ might play an important role in turning on the maturation of follice.%试验旨在探究转化生长因子βⅠ型受体(transforming growth factor-beta receptorⅠ,TGF-βRⅠ)基因在敖汉细毛羊中的组织表达情况,以及在不同发情时期卵巢中表达量的变化规律,为探讨其与绵羊发情的关系奠定基础.以24只季节性发情的敖汉细毛羊为研究对象,分为乏情期、发情间期、发情前期和发情期4组,每组6只.首先利用实时荧光定量PCR检测其发情期甲状腺、下丘脑、垂体、卵巢、肾上腺、心脏、肝脏、脾脏、肺脏、肾脏、胰腺、肌肉12个组织中TGF-βRⅠ基因的表达情况,其次对4个不同发情时期卵巢TGF-βRⅠ基因的表达量变化进行研究.结果显示,TGF-βR Ⅰ在各组织中均有表达,在卵巢和甲状腺中表达量最高,极显著高于其他组织(P<0.01);在下丘脑、垂体、胰腺、肾上腺、脾脏和肺脏组织表达量较高,显著高于心脏、肝脏、肾脏和肌肉组织(P<0.05).卵巢中TGF-βRⅠ基因在发情前期表达量最高,极显著高于其他3个时期(P<0.01),发情间期表达量最低,极显著低于其他3个时期(P<0.01).综上所述,卵巢组织中TGF-βRⅠ基因在发情前期时可能对排卵前卵泡的成熟起促进作用.