不同环境条件对韭菜水溶性浸提液抑藻效果的影响

摘要

[Objective] The paper was to study the algicidal effect of water-soluble extracts of Chinese chive under different environmental conditions, so as to provide reference for further study and development of new algicidal substances. [ Method ] The effects of water-soluble extracts of Chinese chive on the growth of Microcystis aeruginosa under different pH, light and aeration conditions were compared and studied. [ Result] The growth inhibition rate of water-soluble extracts of Chinese chive on M. Aeruginosa was greater than 90% under different pH conditions. With the growth of M. Aeruginosa, the culture liquid with different initial pH was finally tended to 9 -9.5. The growth inhibition rate of water-soluble extracts of Chinese chive on algae cell increased with the prolongation of culture time within the light intensity range of 1 000 - 4 000 lx. The inhibition effect of water-soluble extracts of Chinese chive on M. Aeruginosa under low light intensity (1 000 lx) was better than that under high light intensity, the best light intensity for growth was not conducive to the exertion of allelqpathic effect. Meanwhile, aeration condition was more conducive to the inhibition effect of water-soluble extracts of Chinese chive on the growth of M.' aeruginosa. [ Conclusion] pH, light and aeration conditions all affected the inhibition effect of water-soluble extracts of Chinese chive treated by high temperature on M. Aeruginosa. Understanding the effect of these environmental factors on algicidal effect of allelochemicals could provide reference for further study and development of new algicidal substances.%[目的]研究不同环境条件对韭菜水溶性浸提液抑藻效果的影响,为进一步研究和开发新的抑藻物质提供参考.[方法]通过设置不同的pH、光照强度以及曝气与否,对不同条件下韭菜浸提液对铜绿微囊藻生长的影响进行了对比研究.[结果]在7d的培养过程中,不同pH条件下,韭菜浸提液对铜绿微囊藻的生长抑制均达到90％以上,随着铜绿微囊藻的生长,不同起始pH的培养液,最终的pH趋向于9～9.5之间；在1 000～4 000 lx的光强范围内,韭菜浸提液对藻细胞的生长抑制率均随培养时间的延长而增加,低光照强度(1 000 lx)下,韭菜浸提液对铜绿微囊藻抑制效果要好于高光照强度处理,即最佳的生长光照强度并不有利化感作用的发挥；同时,曝气相对非曝气条件而言,曝气条件更有利于韭菜浸提液对铜绿微囊藻生长的抑制.[结论]该研究结果表明pH、光照强度、是否曝气均影响韭菜水溶性浸提液高温处理后对铜绿微囊藻的抑制效果.探讨这些环境因子对植物化感物质抑藻效果的影响,可为进一步研究和开发新的抑藻物质提供参考.