海蒿子脂溶性物质的抗食物腐败菌活性研究

摘要

[ Objective] To study the antimicrobial activities of liposoluble compounds from Sargassum pallidum (tvrn) Ag against food spoilage bacteria. [ Method] Using the circle agar disc diffusion method,antimicrobial activities of the liposoluble compounds and fractions isolated by column chromatography from Sargassum pallidum (tvrn) Ag were assayed against food spoilage bacteria. [ Result ] The inhibition zone of Sargassum pallidum( tvrn) Ag crude grease against Escherichia coli and Pseudomonas stutzeri was 1.0 and 1.5 mm. Bacillus subtilis and Staphylo-coccus aureus were not sensitive to crude grease. Benzene elution fraction had no antimicrobial activities against Escherichia coli and Pseudomonas stutzeri,and its inhibition zone against Staphylococcus aureus and Bacillus subtilis was both 1.0 mm. Petroleum ether elution fraction all had no antimicrobial activities. Ethanol elution fraction showed strong antimicrobial activities against four food spoilage bacteria and the inhibition zone against Bacillus subtilis,Escherichia coli,Staphylococcus aureus and Pseudomonas stutzeri reached 3. 0,4. 5,4. 0,and 2. 5 mm. In addition, the blank control group had no antimicrobial activities. [ Conclusion ] Ethanol elution fraction of Sargassum pallidum (tvrn) Ag showed strong antimicrobial activities against four food spoilage bacteria. The antimicrobial activities of liposoluble compounds were attributed to their strongly polar lipids.%[目的]研究海蒿子脂溶性物质的抗食物腐败茵活性.[方法]采用圆形纸片法,对海蒿子脂溶性物质及经柱层析分离得到的不同洗脱组分进行抗食物腐败茵试验.[结果]海篙子粗脂对大肠杆菌和假单胞杆菌的抑茵圈分别为1.0和1.5 mm;枯草芽孢杆菌和金黄色葡萄球菌对海蒿子粗脂不敏感.苯洗脱组分对金黄色葡萄球菌与枯草芽孢杆菌抑茵圈均为1.0 mm;苯洗脱组分对大肠杆菌和假单胞杆菌无抑茵活性；石油醚洗脱组分对4种腐败茵亦无抑茵活性；乙醇洗脱组分对枯草芽孢杆菌、大肠杆菌、金黄色葡萄球菌和假单胞杆茵的抑茵圈分别为3.0、4.5、4.0和2.5mm,空白对照组均无抑茵活性.[结论]海蒿子乙醇洗脱组分具有较强的抗茵活性;多数海藻脂溶性物质的抗茵活性主要体现在其强极性脂成分上.