[目的]对金铁锁居群在水分限制下的叶解剖结构进行研究.[方法]选取引种至实验大田的多年生草本植物金铁锁的2年生实生苗为研究对象,在给水和不给水2种条件下,采用常规石蜡切片法和印迹法,对2个居群的叶解剖结构及气孔的生理特性进行了对比研究.[结果]在冬春季干旱期,不给水栽培条件下的金铁锁表现为叶片上角质层变厚,栅栏组织和海绵组织比值增加,维管束直径增加,主脉木质部维管数增加；叶气孔口变小,气孔密度增加等变化,可以将这些性状的可塑性作为金铁锁居群对水分亏缺作出反应的重要指标.同时,这些指标的变化幅度存在居群间差异.丽江阿西居群在不给水的栽培条件下,叶解剖结构和气孔特性变化显著,从而可能使该居群对干旱环境具有更强的适应能力.[结论]对筛选抗旱金铁锁种质资源及探讨居群在自然干旱条件下的适应性分化具有参者价值.%[Objective] This study aimed to study the leaf anatomical structures of P. Tunicoides populations under water-limited conditions. [ Method ] 2-year-old seedlings of herbaceous perennial P. Tunicoides that was introduced into the experimental field were selected as the research subject. Under the well-watered and water-limited conditions, we conducted the comparative observation of leaf anatomical structure and stomatal characteristics of two populations by using paraffin slice method and imprinting method. [ Result ] The result showed that the upper cuticle of P. Tunicoides leaves were thickened, the palisade tissue / spongy tissue ratio, vascular bundle diameter and number of xylem vessels in main vein had increased, the stomatal opening became smaller and the stomatal density had increased in winter-spring drought period under water-limited conditions. The plasticity of above traits could be regarded as the important indicators for detecting the responses of P. Tunicoides populations to water-limited conditions. Meanwhile, variation amplitudes of these indicators were different among populations. Leaf anatomical structure and stomatal characteristics in Lijiang Axi population under water-limited conditions varied significantly, which might cause the stronger adaptability of this population to drought conditions. [ Conclusion ] This research provided a reference for the selection of drought-resistant P. Tunicoides germplasm and exploration of the adaptive differentiation of P. Tunicoides populations under natural drought conditions.
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