长双歧杆菌NCC2705分泌蛋白的全基因组预测和功能分析

摘要

With the genome proteins sequences of Bifidobacterium longum FNCC2705 as study objects,SignalP 4.0,LipoP,TMHMM 2.0 software were used to analyze the secretory proteins and types of signal peptide.Function of secretory proteins was also analyzed by COG ( Cluster of Or-thologous Groups of proteins) database.Results showed a total of 37 secretion proteins through Sec pathway which included 27 secreted proteins recognized by SPaseⅠand 10 secreted proteins recognized by SPaseⅡ.Maximum length of their signal peptide is 52 aa and minimum length is 10 aa respectively.The functions of Bifidobacterium secretory protein showed there were many hypothetical proteins.Secretory protein with annotation of COG functions were mainly involved in amino acid transport and metabolism,carbohydrate transport and metabolism,inorganic ion transport and metabolism,etc..The functions of these secretory proteins can help bifidobacterium adapt to its host environment.%以长双歧杆菌NCC2705基因组序列为研究对象，使用SignalP 4．0、LipoP、TMHMM 2．0软件分析该基因组中的分泌蛋白及其信号肽的类型，同时采用COG （ Cluster of Orthologous Groups of proteins）功能数据库对预测的分泌蛋白进行功能注释和聚类分析。结果表明，长双岐杆菌NCC2705中共有37个Sec途径分泌蛋白，其中Sec途径分泌蛋白包括27个被I型（ SPaseⅠ）信号肽酶和10个Ⅱ型信号肽酶（ SpaseII）识别的蛋白，Sec 途径分泌蛋白信号肽长度最多的有52个氨基酸，最少的有10个氨基酸。分泌蛋白的功能分析表明，该菌株分泌蛋白中含有大部分的假定蛋白，主要参与氨基酸代谢与转运、碳水化合物代谢转运，无机盐离子代谢转运，细胞壁和细胞膜生物合成的功能有关。