高效液相色谱法测定通便和减肥类保健食品中橙黄决明素的含量

摘要

[目的]建立高效液相色谱法测定通便和减肥类保健食品中主要功效成分决明子中橙黄决明素的含量。[方法]色谱条件：采用C18柱，柱温30℃，检测波长284 nm，流速为1 mL／min，乙腈－0．1％磷酸溶液（40∶60）为流动相。[结果]橙黄决明素在0．0402～0．3216μg（r＝0．9999）范围内呈良好的线性关系，平均加样回收率为99．87％，RSD为1．32％。[结论]该试验所建立的方法准确、重现性好，可用于通便和减肥类保健食品的质量控制。%Objective] To set up a simple and accurate method to determine the aurantio-obtusifolin content in health food for catharsis and weight loose by HPLC.[Method] Chromatographic condition was as follows:C18 column was adopted;column temperature was 30℃, the detec-tion wavelength was 284 nm, flow rate was 1 mL/min, mobile phase was acetonitrile -0.1%phosphoric acid solution (40∶60).[Result] Au-rantio -obtusifolin showed good linear relationship within the range of 0.040 2 -0.321 6 μg (r =0.999 9).The average recovery rate was 99.87% and RS D was 1.32%.[ Conclusion] The established method is accurate, reproducible and can be used for quality control of health food for catharsis and weight loose .