黑豆不同部分馏油的体外抗氧化性比较

摘要

[目的]通过体外抗氧化体系比较黑豆不同部分馏油抗氧化性活性.[方法]通过正交试验优化索氏提取黑豆馏油的最佳工艺,提取黑豆不同部分馏油;研究黑豆不同部分馏油对羟基自由基(·OH)、DPPH自由基(DPPH·)的清除能力.[结果]在样品浓度为1.0 mg/mL时,黑豆不同部分馏油对·OH的清除率分别为全豆馏油83.9%、豆黄(黑豆去皮部分)馏油64.8%、豆皮馏油17.3%,对DPPH·的清除率分别为全豆馏油41.3%、豆黄馏油33.2%、豆皮馏油77.9%.[结论]黑豆不同部分馏油均具有较好的抗氧化性,是良好的天然抗氧化剂.黑豆不同部分馏油对·OH的清除能力从大到小依次为全豆馏油、豆黄馏油、豆皮馏油,对DPPH·的清除能力从大到小依次为豆皮馏油、全豆馏油、豆黄馏油.%[Objective]This research aimed to study the antioxidant activity of distillation oil of different parts of black beans in different antioxidant systems separately.[Method]The optimum process of the extraction of black beans distillate oil was optimized by orthogonal test,the distillation oil from different parts of black beans were extracted.The scavenging ability of hydroxyl radical (·OH) and DPPH free radical (DPPH·) in distillation oil of different parts of black beans were studied.[Result]When the concentration of the sample was 1.0 mg/mL, the removal rate of different parts of the black beans distillation oil to ·OH was 83.9% of the whole bean distillate oil, 64.8% of beans without peel and 17.3% of bean peel, the removal ability of DPPH· was 41.3% of the whole bean distillate oil, 33.2% of beans without peel and 77.9% of bean peel.[Conclusion]Distillation oil of different parts of the black beans has strong antioxidant capacity and it is a good natural antioxidant.The black beans different parts of distillation oil on the ·OH scavenging ability: whole bean>bean without peel>bean peel;DPPH· scavenging ability: bean peel> whole bean> bean without peel.