毒死蜱降解菌的筛选·鉴定·降解特性

摘要

[Objective]To screen chlorpyrifos degrading bacteria and understand their characteristics.[Method]A strain capable of utilizing chlorpyrifos as the sole carbon and energy sources was isolated form Chlorpyrifos used paddy soil.[Result]The results showed that the degradation rate of Chlorpyrifos was at 83.3% in liquid culture medium with 15 days.The phylogenetic analysis and 16S rDNA gene sequence analysis were applied to the bacteria classification,the strain was identified as Bacillus subtilis.The phylogenetic analysis also showed that the genetic relationship between strain DC1 and Bacillus tequilensis was propinquity.[Conclusion]The degrading bacteria DC1 was derived from soil and has strong adaptability,and has certain application value for solving soil poisoning ticks residue.%[目的]筛选毒死蜱降解菌,了解其特性.[方法]从常年施用毒死蜱农药的水稻田土壤中筛选出1株能以毒死蜱为唯一碳源和能源的降解菌.[结果]降解菌DC1对浓度100 mg/L毒死蜱15 d的降解率可达到83.3%.通过16S rDNA序列同源性和系统发育分析,将该毒死蜱降解菌鉴定为枯草芽孢杆菌(Bacillus subtilis).系统发育表明,该菌和枯草芽孢杆菌的分支特基拉芽孢杆菌(Bacillus tequilensis)的亲缘关系最近.[结论]降解菌DC1来源于土壤,适应性强,对解决土壤中毒死蜱残留有一定的应用价值.