龙胆中黄酮化合物含量测定的方法学研究

摘要

[目的]建立紫外可见分光光度法测定龙胆中总黄酮含量的方法.[方法]通过微波辅助双水相提取龙胆中总黄酮类化合物,采用紫外可见分光光度法,以芦丁作为对照品,测定其含量.[结果]黄酮类化合物浓度在10.4~73.0 μg/mL,其吸光度与浓度有良好的线性关系;该方法的精密度、重复性和加样回收率的RSD值分别为0.30%、1.31%、2.27%.[结论]该方法准确、快速、重复性好,可以用于测定龙胆中总黄酮类化合物.%[Objective] The research aimed to establish a method for determination of the total flavonoids from Radix Gentianae.[Method]The total flavonoids in Radix Gentianae were extracted by microwave-assisted aqueous two-phase.The contents of total flavonoids were determined by UV-visible spectrophotometry with rutin as the reference substance.[Result]The method was a good linear at the range of 10.4-73.0 μg/mL of the total flavonoids.The RSD values of the precision test, reproducibility experiment and the recovery rate test were 0.30%,1.31%,2.27%,respectively.[Conclusion]The method is accurate, rapid and reproducible,and can be used to determine the total flavonoids from Radix Gentianae.