紫丁香苷抗肿瘤活性筛选及作用机制研究

摘要

[Objective]To screen human tumor cell lines sensitive to syringin, and study its anticancer mechanism. [Method]To determine the inhibitory effect of syringin in vitro using tetrazolium salt(MTT) assay,and study the role of apoptosis pathway by assay of Caspase-3 ac-tivity. [Result]Among the 15 common human tumor cell lines,syringin IC50of hepatoma cell HepG2 and prostate cancer cell PC-3 were below 100 μg/mL. Syringin can promote the activity of Caspase-3 in tumor cells. [Conclusion] Hepatoma cell HepG2 and prostate cancer cell PC-3 are more sensitive to syringin,and the inhibition of syringin presents a dose-dependent manner. The cell apoptosis pathway plays a role in the anticancer activity of syringin.%[目的]筛选对紫丁香苷较敏感的人肿瘤细胞系,研究其抗癌机理.[方法]采用四氮唑盐法(MTT法)测定药物的体外抑制作用,通过测定Caspase-3活性研究细胞凋亡通路在抗癌活性中的作用.[结果]在常见的15种人肿瘤细胞中,紫丁香苷的半数抑制浓度IC50低于100 μg/mL的有人肝癌细胞HepG2以及人前列腺癌细胞PC-3,紫丁香苷可促进肿瘤细胞中细胞凋亡因子Caspase-3活性增加.[结论]人肝癌细胞HepG2以及人前列腺癌细胞PC-3对紫丁香苷较为敏感,紫丁香苷的抑制作用呈现出剂量依赖的特点;细胞凋亡途径在紫丁香苷的抗肿瘤活性中起着一定的作用.