The current study was aimed to analyze the association of promoter Ⅲ in Acetyl-CoA carboxylases-ct gene (ACACA) with milk traits in Laoshan dairy goat (Capra hircus). Denaturing high-performance liquid chromatography (PCR-DHPLC) and direct sequencing were applied to identify SNPs in promoter Ⅲ of ACACA gene in Laoshan dairy goat population containing 174 samples, and least square method was performed to evaluate the association of polymorphism in promoter HI with milk traits. The SNPs have been identified as followed, B/D at 1206 bp, A/C at 1255 bp, M/N at 1322 bp. The distribution of alleles and genotypes in both Group F and Group P was not balanced, but still under Hardy-Weinberg equilibrium. The variation between two genotypes was not significant in the whole population, only the difference between different herbs existed. Further, the difference between different genotypes were analyzed in Group F and Group P, respectively. The 1206 site in F group, allele B had a positive effect on protein , lactose and non-fat rate, and the difference between genotypes BB and BD was statistically significant for protein rate and fat rate (P<0.01), for non-fat and milk density (P<0.05). The substitution effects for protein, lactose and non-fat rate were significant (F<0.05). While in P group, the difference between genotypes BD and BB for all milk traits was not significant (P>0.05). Allele N was the dominant allele for lactose rate, while this was the negative allele for milk yield at 1322 site in F group, and the difference between genotypes MN and MM was significant for milk yield (P< 0.01) and for lactose rate (P<0.05). The substitution effects for lactose rate and milk yield were 0.12% and -52.22 kg (P<0.0l), respectively. In P group, allele M was the dominant allele for protein rate, lactose rate, non-fat and milk density, the difference between genotypes MM and MN was significant for protein rate, lactose rate, non-fat and milk density (P<0.05). The substitution effects for lactose rate and non-fat were -0.25%, -0.44% (P<0.05), respectively. This study shows that the same alleles of promoter P HI in ACACA gene vary in their effects in different breeding groups, for milk protein rate, lactose rate, non-fat material rate, milk density and 300 d milk yield.%利用高效液相色谱法(denaturing high-performance liquid chromatography,DHPLC)和直接测序法,检测了174只崂山奶山羊(Capra hirc us)泌乳母羊的ACA CA基因启动子PⅢ位点多态性,采用最小二乘方法分析其与泌乳性状之间的关联效应.结果显示,在启动子PⅢ部分序列中共检测到3个SNPs,即第1206位的B/D,1255位的A/C和1322位的M/N,其等位基因和基因型频率在种羊场(F)和农户(P)两个群体中分布不均衡,均处于Hardy-Weinberg平衡状态(P＞0.05).在两个样本群中,大部分泌乳性状均存在显著的场别效应(P＜0.01或P＜0.05),而位点的基因型效应均不显著(P＞0.05).在F群体的1206位点上,等位基因B为乳蛋白率、乳糖率、非脂肪物性状的有利等位基因,BB基因型与BD基因型的乳蛋白率和乳糖率性状差异极显著(P＜0.01),非脂肪物和乳密度性状差异显著(P＜0.05),乳蛋白率、乳糖率和非脂肪物性状均存在显著的等位基因替代效应(P＜0.05)；而在P群体中,两种基因型之间的所有性状则差异不显著(P＞0.05).在F群体的1322位点上,等位基因N为乳糖率的有利等位基因,为300 d产奶量的不利等位基因,MN基因型与MM基因型的300 d产奶量和乳糖率性状差异显著(P＜0.01或P＜0.05),其等位基因替代效应分别为-52.22 kg和0.12％(P＜0.01)；而在P群体中,等位基因M为乳蛋白率、乳糖率、非脂肪物、乳密度的有利等位基因,基因型MM与MN在乳蛋白率、乳糖率、非脂肪物和乳密度性状均存在显著差异(P＜0.05),乳糖率和非脂肪物的等位基因替代效应分别为-0.25％和-0.44％(P＜0.05).研究结果提示,ACACA基因启动子PⅢ相同等位基因在不同育种群体中,对乳蛋白率、乳糖率、非脂肪物、乳密度、300 d产奶量存在不同程度的影响.
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