Kit for detection of SNPs in promoter III region of acetyl-CoA carboxylase (ACACA) gene in sheep

摘要

The solution concerns the set for the detection of SNPs in the promoter III of the acetyl -CoA carboxylase (ACACA) gene range in ovine polymer a se chain reactions at the genomic DNA level and subsequently by mini -sequencing, SnaPshot,which is characterized by containing examples of the composition of ov -ACACACA F: 5 -ggg CCC CTT TAC gTT TCT gT -3, ov -ACACA R: 5 -AgC TTC TgC CTT AgC TgC A -3 with amplicon length 513 bp,and a n extended example for the detection of new genetic polymorphism in position 1168 bp A /G o n the composition of Ov -acaca Sh1: 5 - (AT).sub.2.n.gTg gTg gCg TgT CTC -3'.The set also contains a set of extended examples of the composition of Ov -acaca Sh2: 5 - (AT).sub.3.n.gCA TAg CTg TCC TCg TAA gC -3, Ov -acaca Sh3: 5 - (AT).sub.6.n.gAA ggC TCA ggg CAA AAA gA -3, Ov -acaca Sh4: 5 - (AT).sub.8.n.gAC CgT CAg AAg AAA gTg AC -3 for the multiple detection of genetic variants in positions 1330 bp G /T, 1338 bp C /G and 1430 bp C /T.