Soybean cyst nematode (SCN) is a pest which can cause worldwide soybean yield losses seriously. The resistance of soybean to SCN is controlled by multiple genes. It is very important to detect the disease-resistant genes and breed the SCN disease-resistant cultivar. In order to understand the relationship between the gene and resistance to SCN, we analyzed the expression characteristics of candidate gene GmHslpro-1 on Soybean (Glycine max) resistant line Zhongpin03-5373 and susceptible eultivar Zhonghuang 13 using the Real-time PCR technique.We found that the relative expression of the gene raised after 1 d vaccinating SCN 4 Race (SCN 4) and it was 13.4 times higher than that of the control sample, it kept upward until inoculated 6 d. Analysis of variance showed that the relative expression of the gene was significant difference between inoculated and un-inoculated at significant difference (0.05 or 0.01 level). It indicates that GmHslpro-1 may be induced to express in the conditions of SCN 4 infection, and the gene has a certain resistance to SCN 4.%大豆孢囊线虫(Heterodera glycines,Soybean cyst nematode,SCN)是严重危害世界大豆生产的害虫,大豆对孢囊线虫的抗性由多个基因控制,发掘和利用抗病基因对于SCN抗病品种的有效选育至关重要.本研究以大豆(Glycine max)感病品种中黄13和抗病品系中品03-5373为研究材料,对包含线虫抗病基因相关结构域Hs1pro-1_N和Hs1pro-1_C的候选基因GmHs1pro-1的表达特征进行实时荧光定量PCR分析,以期明确该基因与SCN抗性之间的关系.研究发现接种SCN 4号生理小种(SCN 4)后1 d该基因的相对表达量出现明显的上调,比对照样品高13.4倍,其后的各时间点其表达量仍然保持持续上调的趋势,在接种6 d后其上调趋势有所减弱.方差分析表明,接种与未接种相对表达量之间的差异均达到显著(P<0.05)和极显著(P<0.01)水平,说明SCN 4侵染条件下,GmHs1pro1基因被诱导表达,该基因对SCN 4具有一定的抗性作用.
展开▼
