由于转基因标准物质的缺乏,无法保证检测结果的准确、可靠和可比.本研究将筛选后的转基因玉米(Zea mays)NK603阳性材料及受体进行研磨筛分,粒径测定结果表明,研磨后的粒径大小为100 μm左右.采用重量法配置了3个不同水平的转基因玉米基体物质,应用实时荧光定量PCR方法对研制的标准物质进行均匀性和稳定性检测,统计结果表明,所研制的基体标准物质均匀性良好;在4℃保存条件下,一年内未发现不稳定现象.重量法确定的标准值和扩展不确定度(k=2)分别为(0.50±0.10)％、(1.00±0.35)％和(5.00±0.35)％.同时选择7家实验室进行协同验证,结果表明,研制的转基因玉米NK603基体标准物质量值准确可靠,与国外标准物质相比,不确定度水平相当.本实验研制的转基因玉米NK603基体标准物质适用于转基因玉米NK603的定量检测.%The accuracy, reliability and comparability of genetically modified (GM) detection result can not be ensured due to the lack of GM reference material. In this paper, the selected genetically modified (GM) maize (Zea mays) line NK603 and its recipient were grinded and the particle size was about 100 μm. Three different levels of matrix reference materials were gravimetrically prepared. The homogeneity and stability were checked by quantitative real time PCR. The result showed the matrix reference materials were homogenous and stable under the 4°C during one year's stock. The property values determined by gravimetric method and their expanded uncertainties (k=2) of three matrix GM reference material were (0.50+0.10)%, (1.00±0.35)% and (5.00±0.35)%, respectively. Seven different laboratories were chosen to validate the property values of the GM maize reference materials and the result showed that it w as agreed well with the value determined by the gravimetrical method for each level. The uncertainties of the GM reference materials were comparable with those international GM reference material. Thus, the GM maize reference materials can be applied for the GMrnmaize quantitative detection to ensure the detection result accurate and reliable.
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