白芍总苷对阿霉素肾病大鼠肾脏的保护作用

摘要

Objective To investigate the protective effect of total glucosides of paeony(TGP)on kidney of rats with adria-mycin nephropathy(AN).Method A total of 40 SD male rats were randomly assigned into normal control group,untreated group,low dose TGP group,medium dose TGP group and high dose TGP group.Except normal control group,rats were injected via caudal vein with adriamycin to induce nephropathy.Then the TGP was given with lavage AN rats in the treatment groups, while replaced saline was given to rats in normal control group and untreated group,for 8 successive weeks.At the end of the ex-periment,the 24 hour urinary protein quantitation (24 h UPQ),serum albumin (ALB),total cholesterol (TC),triglyceride (TG),serum creatinine(SCr)and blood urea nitrogen(BUN)were measured,ultrastructure was observed,serum IL -18 and IFN -γwere tested by enzyme linked immunosorbent assay,mRNA expression of IL -18 and IFN -γwere detected by Real-time PCR.Results Compared with normal control group,the significant exaltation in the expression of IL -18 and IFN -γ, 24 h UPQ,TC,TG,SCr,BUN and renal pathological injury were reelvealed in all group,the difference was statistically signifi-cant (P <0.05);which were obviously decreased in TGP group than those in untreated group,the difference was statistically significant (P <0.05 );while there dose dependent in TGP group,the difference was statistically significant (P <0.05 ).Conclusion The total glucosides of paeony can in some extent improve the renal pathological damage which indeced by adria-mycin,its mechanism may be related to regulating the immune function and cytokine balance.%目的：探讨白芍总苷对阿霉素肾病大鼠肾脏的保护作用。方法：除正常对照组8只外，雄性 SD 大鼠32只经尾静脉注射阿霉素建立阿霉素肾病模型。造模2周后将造模成功的大鼠随机分为模型组、白芍总苷低剂量组、白芍总苷中剂量组及白芍总苷高剂量组，各8只，分别予相应的药物灌胃治疗。给药8周后检测各组大鼠24 h 尿蛋白定量、血清白蛋白、总胆固醇、三酰甘油、血尿素及血肌酐水平，光镜观察肾病理变化，ELISA 法定量分析各组大鼠血清中 IL －18、IFN －γ水平，RT －PCR 检测肾组织中 IL －18、IFN －γ的表达。结果：与正常组比较，模型组尿蛋白、血肌酐、血尿素氮、总胆固醇、三酰甘油及 IL －18、IFN －γmRNA 和抗体表达水平明显升高，差异有统计学意义（P ＜0.05），血清白蛋白明显降低，差异有统计学意义（P ＜0.05）；并伴肾小球硬化及小管间质损害；白芍总苷组能降低尿蛋白、血肌酐、血尿素氮、总胆固醇、三酰甘油及 IL －18、IFN －γmRNA 和抗体表达水平，升高血清白蛋白水平，改善肾病理损伤，差异有统计学意义（P ＜0.05）；且白芍总苷治疗效果呈剂量依赖性，差异有统计学意义（P ＜0.05）。结论：白芍总苷能在一定程度上改善阿霉素引起的肾损害，其作用机制可能与调节其免疫功能，平衡细胞因子的产生有关。