目的：探讨样本溶血对酶联免疫吸附试验（ELISA）检测弱阳性乙型肝炎表面抗原（HBsAg）结果的影响及抗干扰措施的效果。方法：将60例 HBsAg 弱阳性（浓度在0.5～1.2 ng／ml 的）样本人为造成轻、中、重度溶血，采用ELISA 检测所有样本的样本对临界值（光谱）比例（S ／CO）值，比较溶血前后样本的 S ／CO 值；对溶血标本增设试验孔，对试验孔抗溶血干扰的效果进行探讨。结果：不同溶血程度样本溶血后及校正后的 S ／CO 值均高于溶血前，前后比较差异有统计学意义（P ＜0.05）；随着溶血程度的加重，对样本检测结果的影响也逐渐增加；60例弱阳性标本经溶血处理后检测结果仍为阳性，经试验孔校正后也仍为阳性。结论：样本溶血对于 HBsAg 弱阳性检测具有一定的干扰作用，通过增设试验孔的方式可部分校正溶血形成的干扰，提高试验的准确性，但是对弱阳性样本的定性判断无影响。%Objective To evaluate the impact of hemolysis on the detection results of HBsAg weak positive by ELISA and the intervention measures.Method 60 samples with HBsAg weak positive (concentration in 0.5 ~1.2 ng/ml)were mild,moderate and severe caused hemolysis.Using ELISA to assay S /CO values of all samples before and after hemolysis.Add additional test wells for all hemolysis samples.The anti -hemolytic interference effects of test wells were discussed.Results The S /CO values of varying degrees of hemolytic samples and test wells were all high than before hemolysis.The differences were all statistically sig-nificant (P <0.05).The influence was increased follow the severity of hemolysis.60 cases of hemolytic still positive,test wells after correction also remained positive.Conclusion Sample hemolysis can interference the detection results of HBsAg weak posi-tive by ELISA.Add additional test wells can be partially corrected hemolysis formed to improve the accuracy of the test,but had no effect on sample qualitative judgment.
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