扶正解毒方对小鼠前胃癌诱导肿瘤相关巨噬细胞的影响

摘要

目的：探讨扶正解毒方对小鼠前胃癌所诱导肿瘤相关巨噬细胞影响性，以期为临床上治疗提供有效依据。方法用75只小鼠随机分成5组，每组15只，均为雄性，分别为空白组、模型组、中药组、化疗组、中西医结合组，空白组灌注生理盐水，其他4组先进行胃癌诱导肿瘤造模，空白组不作任何处理，造模组予生理盐水灌胃3周，中药组予扶正解毒方治疗，化疗组予5-氟尿嘧啶腹腔注射1周，中西医结合组先5-氟尿嘧啶腹腔注射1周后再中药灌胃3周，然后采用相关检测方法检测巨噬细胞相关指标含量。结果 CD68＋巨噬细胞浸润程度上，模型组为3＋，中药组为2＋，化疗和中西医结合组为1＋；M2型巨噬细胞含量中模型组和其他组比较差异均有统计学意义（P <0.05），而化疗组和中西医结合组比较差异无统计学意义（P >0.05）；而在 IL-4、IL-10、IL-13、TGF-β含量上，空白组和其他四组比较差异均有统计学意义（P <0.05），模型组和中药组、化疗组、中西医结合组比较差异均有统计学意义（ P <0.05），而化疗组、中西医结合组比较差异无统计学意义（ P >0.05），中药组和化疗组、中西医结合组比较差异均有统计学意义（P <0.05）。结论扶正解毒方能通过降低CD68＋巨噬细胞浸润程度，减轻 M2型巨噬细胞含量，降低炎症指标 IL-4、IL-10、IL-13、TGF-β含量等对小鼠胃癌起抑制作用。%Objective To study the centralizer detoxification before therapy treatment in mice induced by gastric cancer tumor associated macrophage influence,in order to provide effective basis for clinical treatment. Methods With 75 mice were randomly divided into 5 groups,each group of 15 only,are male,respectively for the control group, model group,the Chinese medicine group,chemotherapy group,combining Chinese and western medicine group,the control group with normal saline,the other four groups to gastric cancer induced tumor model,the control does not make any processing,build module to the physiological saline lavage for three weeks,Chinese traditional medicine group to the centralizer detoxification treatment,intraperitoneal injection of chemotherapy group with 5 - fluorouracil in 1 week,combine traditional Chinese and western medicine group first 5 - fluorouracil intraperitoneal injection of tradi-tional Chinese medicine lavage again after 1 week 3 weeks,then the correlation detection method to detect the contents of related parameters of macrophages. Results CD68 + macrophage infiltration degree,the model group for 3 + ,Chi-nese traditional medicine group of 2 + ,chemotherapy and combine traditional Chinese and western medicine group for 1 + ;M2 macrophage content in model group and other groups comparative differences are statistically significant(P< 0. 05),and chemotherapy group and combine traditional Chinese and western medicine group of comparison dife-rence had no statistical significance(P > 0. 05);In IL- 4,IL10,IL-13,TGF - beta content,control group and the other four groups of comparative differences are statistically significant( P < 0. 05),model group and Chinese medicine group,chemotherapy group,combining Chinese and western medicine group comparative differences are statistically significant(P < 0. 05),and chemotherapy group,there was no statistically significant difference compared with way of Chinese and western medicine(P > 0. 05),Chinese medicine and chemotherapy group,combining Chinese and west-ern medicine group comparative differences are statistically significant(P < 0. 05). Conclusion The centralizer can detoxification by reducing CD68 + macrophage infiltration degree,reduce the content of type M2 macrophages,reduce inflammation index IL- 4,IL 10,IL- 13,TGF- beta content and so on have inhibitory effect on gastric cancer in mice.