Objective To explore the effects of angiopoietin-1(Ang-1) and vascular endothelial growth factor (VEGF165) on the proliferation and apotosis of human gastric cancer cell line MGC-803. Methods MCC-803 was transfected with Ad-GFP ( group B), Ad-Ang-1 ( group C),Ad-VEGF165(group D) or Ad-Ang-1 plus Ad-VEGF165(group E). MGC-803 in group A was blank control. The proliferation and apoptosis of MGC-803 were measured by MTT colorimetry assay and flow counting, respectively. Expressions of Bcl-2 and Bax proteins were measured by Western blot.Results Proliferation of MGC-803 in groups of C,D,E at 24,48 and 72 h were higher than that in groups of B and C(P＜0.05) ,which was higher in group E than that in groups of C and D(P＜0.05).Compared to groups of B and A, cell apoptosis was significantly inhibited in groups of C, D, E,especially in group E, (P＜0.01). Expression of Bcl-2 was increased, but that of Bax decreased, in groups of C,D,E than that in groups of B and A(P＜0.05). Conclusion Ang-1 and VEGF165 can significantly promote the proliferation of MGC-803 and reduce its apoptosis. A combination of Ang-1 and VEGF165 has stronger effect on MGC-803 than Ang-1 or VEGF165 alone.%目的 研究人血管生成素1(Ang-1)、血管内皮生长因子165(VEGF165)以及两种因子相互作用对人胃癌细胞株MGC-803增殖与凋亡的影响.方法 应用MTT法测定腺病毒绿色荧光蛋白(Ad-GFP)(B组)、Ad-Ang-1(C组)、Ad-VEGF165(D组)以及Ad-Ang-1+Ad-VEGF165/2(E组)对MGC-803细胞增殖的影响;另设对照组(A组).采用流式细胞术分析血清饥饿时其对凋亡的影响;运用Western blot方法检测Bc1-2和Bax蛋白的表达.结果 24、48、72 h时,C、D、E组吸光度均明显高于B、A组(P＜0.05);E组吸光度明显高于C、D组(P＜0.05).与A组或B组相比,C、D、E组均能够抑制细胞凋亡(P＜0.01),其中E组抑制凋亡作用最强.C、D、E组Bcl-2蛋白表达均比B、A组明显增高(P＜0.05),Bax蛋白的表达则明显降低(P＜0.05).结论 Ang-1、VEGF165和Ang-1+VEGF165/2能够明显促进MGC-803细胞体外增殖,抑制血清饥饿时的凋亡;Ang-1与VEGF165联合作用要显著强于单用Ang-1或VEGF165.
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