参麦注射液预处理对大鼠心肌缺血-再灌注损伤中胰岛素抵抗的影响

摘要

目的 探讨参麦注射液(SM)对胰岛素(Ins)抵抗是否有改善作用.方法 SD雄性大鼠24只随机均分为两组:A组每天腹腔注射SM 10 ml/kg,连续3 d；B组注射等量生理盐水.末次给药后30 min采用冠脉结扎15 min后再松开45 min的办法制备大鼠心肌缺血-再灌注(I-R)损伤模型.放射免疫法检测血糖(Glu)、Ins和C肽的浓度,ELISA法检测血浆抵抗素和游离脂肪酸(FFA)含量.结果 A组血浆Glu[(3.64±0.53) mmol/L vs.(4.29±0.62)mmol/L]、Ins[(31.68±0.21)μU/ml vs.(39.17±0.69)μU/ml]、C肽[(9.26±1.87)ng/ml vs.(13.05±2.56) ng/ml]的浓度以及血浆FFA[ (326.40±35.63)μmol/L vs.(429.30±29.64)μmol/L]和抵抗素[(13.29±1.16) ng/ml vs.(16.32±1.52) ng/ml](P＜0.05)的含量均明显低于B组(P＜0.05).结论 SM预处理能减轻大鼠心肌I-R损伤过程中的Ins抵抗,对心肌产生一定的保护作用.%Objective To investigate the effects of Shenmai injection(SM) preconditioning on insulin resistance. Methods Twenty-four heathy male SD rats were equally randomized into two groups of ACpretreated with SM 10 ml · kg-1 · d-1 for 3 days) and B(pretreated with equal volume of normal saline as the control). At 30 min after the last administration, ischemia for 15 min and reperfusion for 45 min were performed to establish ischemia-reperfusion (I-R) injury models by ligating the left anterior coronary artery and then loosening. Plasma glucose(Glu), free fatty acid (FFA),serum insulin(Ins),C-peptide and resistin were detected by ELISA and radioimmunoassay. Results The concentrations of Glu [(3. 64±0. 53) mmol/L vs. (4. 29±0. 62) mmol/L],Ins[(31. 68 ±0. 21) fAJ/ml vs. (39.17±0. 69) μU/ml],Opeptide[(9. 26±1. 87) ng/ml vs. (13.05±2. 56) ng/ml], FFA[(326.40±35.63) mol/L vs. (429. 30±29. 64) μmol/L] and resistin[( 13. 29± 1.16) ng/ml vs. (16. 32±1. 52) ng/ml] were all lower in group A than those in group B(P<0. 05). Conclusion SM injection preconditioning can protect the myocardium from I-R injury by attenuating insulin resistance in myocardial I-R rats.