目的：筛选偏头痛大鼠三叉神经节（TG）中调控 IL‐1β的微小RNA（miRNA）。方法34只SD大鼠行矢状窦旁硬脑膜外置管后随机分为假手术组（不用药，n＝13）、注射致炎剂（IS ）10μl／d 1次（IS 1 d组，n＝4）、2次（IS 2 d组，n＝4）和3次组（IS 3 d组，n＝13）。Western blot检测TG中IL‐1β表达。提取假手术组及IS 3 d组大鼠TG的RNA进行miRNA芯片检测；软件预测靶作用IL‐1β的miRNA ，结合芯片结果取交集。筛选5个交集中的miRNA和3个上调的miRNA并进行qRT‐PCR验证。结果与假手术组比较，IS 2 d组和IS 3 d组大鼠T G中IL‐1β升高。IS 3 d组共有23个差异表达的miRNA（倍数＞1.5，上调的有17个，下调的有6个）。qRT‐PCR结果显示，1个上调的miRNA （miRNA‐3560）及2个下调的miRNA（miRNA‐760‐3p、miRNA‐130a‐3p）与芯片结果一致（P＜0．05）。结论 IS 3 d组与假手术组大鼠 TG中存在着差异表达的miRNA ，miRNA‐760‐3p和miRNA‐130a‐3p可能调节IL‐1β的表达，从而参与IL‐1β在偏头痛中的分子机制。%Objective To screen the microRNAs (miRNA ) in trigeminal ganglia (TG ) regulating IL‐1β in rat model with migraine .Methods Parasagittal epidural catheterization was performed in 34 SD rats ,which were randomly divided into four groups of C(sham‐operated ,n=13) , IS1(infusion of inflammation soup 10 μl/d for one day ,n= 4) ,IS2(for 2 days ,n= 4) and IS3(for 3 days ,n=13) .Western blot analysis was used to detect the expression of IL‐1βin TG .The RNA in rat TG in groups of C and IS3 was extracted for detecting aberrant miRNA regulation using the miRNA microarray .Software was applied to predict miRNAs that directly targeted at IL‐1β.Then the predicted miRNAs belonging to down‐regulated miRNAs were validated via qRT‐PCR .Results Compared with group C ,IL‐1βin TG was increased in groups of IS2 and IS3 .Of 23 aberrant miRNAs in group IS3 ,the expressions of 17 miRNAs were up‐regulated and 6 miRNAs were down‐regulated . The results of qRT‐PCR were consistent with those of microarray ,which showed that miRNA‐3560 was up‐regulated and two miRNAs of miRNA‐760‐3p and miRNA‐130a‐3p were down‐regulated (P<0 .05) .Conclusion The differential expression of miRNA in TG exists between groups of IS3 and C .miRNA‐760‐3p and miRNA‐130a‐3p may participate in the molecular mechanisms of migraine by regulating the expression of IL‐1β.
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