目的：了解登革热流行病学特征，分析分离毒株 E 基因分子进化特征。方法采用描述性流行病学方法分析2013年广州市登革热疫情，采用酶联免疫法（ELISA）对登革热疑似病例血清进行抗体检测，阳性病例的急性期血清标本以 C6／36细胞进行病毒分离培养；采用 RT-PCR 扩增分离毒株的 E 基因，并对扩增产物进行序列分析，应用 MEGA 5.05进行进化特征分析。结果2013年广州市累计报告登革热确诊病例1270例，发病率为9.96／10万，以本地病例为主（占98.66％），输入病例以东南亚国家为主（占88.24％）。发病高峰为10－11月（占85.28％）。169份登革热病例急性期血清共分离48株登革病毒（Ⅰ型47株，Ⅱ型1株），与近年来广州、东南亚分离株高度同源。结论广州市登革热发病率呈上升趋势，暴发风险较大，须加强监测力度，强化蚊媒的控制，降低登革热传播风险。%Objective To investigate epidemiological characteristics of dengue fever;to analyze evolutional characteristics of E gene of newly isolated virus strains.Methods Descriptive epidemiological analysis was used to analyze dengue prevalence in Guangzhou in 2013;ELISA was used for serum antibody analysis of suspected patients;serum of patients in acute phase were inoculated to C6/36 cells for virus isolation;E gene of isolated strains was amplified by RT-PCR,the amplification products were subjected to sequence analysis;MEGA 5.05 was used for evolutional analysis.Results In 2013,1 270 confirmed dengue fever patients were reported in Guangzhou,resulting incidence of 9.96/105 .Local patients accounted for 98.66%,88.24% of imported patients were from Southeast Asia.85.28% were reported in Oct and Nov.Among 169 serum samples subjected to virus isolation,48 strains were successfully isolated,of which 47 strains were serotype I and 1 strain was serotype II.All iso-lated strains were highly homologous with strains isolated in Guangzhou or Southeast Asia.Conclusion The prevalence of dengue fever demonstrated a rising trend in Guangzhou.The risk of dengue outbreaks was rising continuously.Surveillance should be strengthened and mosquito vectors should be controlled more effectively to eliminate the spreading risk of dengue vi-rus.
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