苏钟猪个体身份SNP识别的数字条形码编制

摘要

Suzhong swine is a hybrid breed derived from Taihu sows and Landrace boars. To identify individual Suzhong swine and trace the source of pork product, 52 single nucleotide polymorphisms ( SNP) were developed in the Suzhong swine genomic DNA fragments using 7 pairs of primers out of 29. Among the SNPs, 41 with heterozygosities more than 0. 1 were selected for Suzhong pigs swine genotyping. By converting the genetic loci with sequences of AA, TT, GG, CC, AT, AG, AC, TG, TC, and GC into digital number of 0, 1, 2, 3, 4, 5, 6, 7, 8, and 9, respective-ly, 96 digital barcodes, unique for each individual pig produced, were generated for 96 Suzhong swine derived from 7 boars and 12 sows. Theoretically, the barcodes based on 41 SNPs amplified by the 7 primer pairs can be used for distinguishing about 5 000 000 individual pigs.%苏钟猪是江苏省农业科学院利用太湖猪为母本,长白猪为父本育成的品种。为苏钟猪个体身份识别及猪肉产品溯源之需,对苏钟猪个体身份 SNP识别进行研究。本研究共设计29对引物,并从中筛选7对引物用于苏钟猪的个体身份识别。7对引物扩增产物能直接测序且测序图谱背景干净,序列阅读无歧义。7对引物共扩增52个SNP位点,经关联性分析及杂合度过滤(H≥0.1)后,选留41个 SNP位点用于苏钟猪个体身份识别的数字条形码编制；同时,编制了7头公猪和12头母猪所生的96头苏钟猪个体身份 SNP识别的数字条形码,数字条形码很好地区分了96头苏钟猪的个体身份。根据96头苏钟猪条形码编制时分离到的各引物对扩增片段的基因型数,7对引物扩增的41个 SNP位点,理论上可用于近5.00×106头猪的个体身份识别。因此,本研究建立的苏钟猪个体身份SNP识别的数字条形码编制方法,可用于规模猪场苏钟猪的个体身份识别及肉产品溯源。