Digital Barcode Development for Single Nuclotide Polymorphism （SNP ） Identification of Suzhong Swine Individuals

摘要

Suzhong swine is a hybrid breed derived from Taihu sows and Landrace boars. To identify Suzhong swine individuals and trace the source of pork products,single nucleotide polymorphisms( SNPs) identification of Suzhong swine individuals was studied. A total of 29 pairs of primers were designed and seven pairs of primers were used for identification of Suzhong swine individuals. The products amplified by seven pairs of primers could be directly sequenced,with clean sequencing map background and no ambiguity in sequence read. Totally 52 SNPs loci were amplified by seven pairs of primers,and 41 SNPs loci were reserved for identification of Suzhong swine individuals through correlation analysis and heterozygosity filtration( H≥0. 1). Meantime,the digital barcodes for SNP identification of 96 individuals of Suzhong swine derived from seven boars and 12 sows were developed,which well distinguished 96 individuals of Suzhong swine. Theoretically,41 SNPs amplified by seven pairs of primers could be used for identification of 5. 0 × 10~6 pig individuals. Therefore,digital barcode development method for SNP identification of Suzhong swine individuals can be used for individual identification of Suzhong swine in scale pig farm and meat product traceability.