桃蚜取食和机械损伤对番茄和辣椒PAL、LOX和PPO活性的诱导作用

摘要

为了明确昆虫取食诱导的受害寄主植物与邻近异种健康植物间的化学通讯作用,以番茄和辣椒2种寄主植物为对象,通过测定虫害及机械损伤处理植株及邻近对照异种健康植株的苯丙氨酸解氨酶( PAL)、脂氧合酶( LOX)和多酚氧化酶( PPO)的活性,研究了桃蚜取食和机械损伤诱导的2种寄主植物间与防御相关的化学通讯作用。结果表明,桃蚜取食和机械损伤不仅能诱导番茄和辣椒PAL活性增加,还能诱导邻近异种健康植株PAL活性增加；桃蚜取食和机械损伤能诱导受害植株的LOX和PPO的活性增加,但邻近异种健康植株的LOX和PPO活性没有变化。因此,PAL、LOX和 PPO在桃蚜取食和机械损伤诱导的辣椒和番茄的防御反应中起作用,而PAL在桃蚜取食和机械损伤诱导的番茄-辣椒间的通讯中起作用。%In order to clarify plant communication induced by herbivory, green peach aphid-tomato-pepper and green peach aphid-pepper-tomato as interaction system were chosen to detect the activities of phenylalanine ammonia-lyase ( PAL) , lipoxygenase ( LOX) and polyphenol oxidase ( PPO) in tomato and pepper under different treatments including aphid-feeding pepper ( tomato) , mechanical damage pepper ( tomato) and its neighboring heathy seedlings tomato ( pepper) . The results showed that the activities of PAL were greatly induced in not only mechanical wounding and herbivory seedlings but also their neighboring healthy seedlings. The activities of LOX and PPO were increased in mechanical wounding and herbivory tomato and pepper seedlings, while the activities of LOX and PPO were not in-duced in the receiver seedlings. These results suggested that PAL, LOX and PPO played a role in pepper and tomato defense responses induced by green peach aphid feeding and mechanical damage, while PAL played an important role in the communication between tomato and pepper induced by green peach aphid feeding and mechanical damage.