抗番茄黄化曲叶病基因ty-5序列变异及表达分析

摘要

Tomato yellow leaf curl virus( TYLCV) disease is one of the important diseases of tomato. Breeding of re-sistant varieties was an important way to defense TYLCV disease. In this study, ty-5 gene which rarely reported was cloned and its fragment sequence was analyzed. Sequence mutation happened in the promoter region and the transcribed region of ty-5. Resistant material AVTO1227, which containing the ty-5 gene, was used for expression analyzing of ty-5. The results showed that the expression level of ty-5 and Ty-5 did not have significant change before and after TYLCV inoculation in dif-ferent tomato materials. This study provided theoretical basis for the next step research of the single nucleotide in the tran-scription region of ty-5. In the meantime, linkage marker of ty-5 also provided an effective method for breeding.%番茄黄化曲叶病是番茄的主要病害之一,选育抗病品种是抵御该病害的重要途径.本研究对目前应用较少的抗番茄黄化曲叶病基因(ty-5)进行研究,对克隆片段进行序列分析,确定该基因在启动子区域和转录区域均存在碱基突变,并利用含有该基因的抗病材料AVTO1227对ty-5的表达进行分析,不同材料中ty-5及其等位基因Ty-5的表达量在接种番茄黄化曲叶病毒(TYLCV)前后均没有显著变化.本研究为下一步针对ty-5翻译区域内单核苷酸变异导致其功能变化的研究提供了依据,同时也为抗番茄黄化曲叶病育种提供了有效的连锁标记.