辣木籽对大鼠心肌缺血再灌注损伤的保护作用

摘要

Objective To explore the protective effect of moringa on myocardial ischemia reperfusion injury in rats.Methods 75 SD rats were selected and divided into a sham operation group,a model group,a high dose group,a middle dose group,and a low dose group.The sham operation group and the model group were given the same volume of physiological saline by gavage and the high dose group,the medium middle dose group,and the low dose group moringa seed suspension liquid 0.5 g/kg,1.0 mg/kg,and 2.0 g/kg by gavage,respectively.Myocardial ischemia reperfusion injury model was made;the ischemia time was 30 minutes;and the reperfusion time was 120 minutes.Myocardial infarction area was measured.The levels of serum lactate dehydrogenase,isoenzyme of creatine kinase,malondialdehyde,and superoxide dismutase were detected.Results The myocardial infarction area was larger in the model group than in the sham operation group (P＜0.05).The area of myocardial infarction was lower in the high dose group,the middle dose group,and the low dose group than in the model group (P＜0.05).The activities of lactic acid dehydrogenase,creatine kinase,and superoxide dismutase enzyme and the malondialdehyde content in the high dose group,the middle dose group,and the low dose group were statistically different from those in the model group (P＜0.05).Conclusion Moringa seed has protective effect on myocardial ischemia reperfusion injury in rats,which may relate to its antioxidant effect.%目的 探讨辣木籽对大鼠心肌缺血再灌注损伤的保护作用.方法 选择SD大鼠75只,分为假手术组、模型组、高剂量组、中剂量组、低剂量组,假手术组和模型组分别给予同体积生理盐水灌胃,高中低剂量组分别给予辣木籽混悬液0.5 g/kg、1.0 mg/kg、2.0 g/kg灌胃.制作心肌缺血再灌注损伤模型,缺血30分钟,再灌注120分钟.观察心肌梗死范围,测定血清乳酸脱氢酶、磷酸肌酸激酶、丙二醛、超氧化物歧化酶水平.结果 模型组梗死区心肌梗死范围大于假手术组,高中低剂量组梗死区心肌梗死范围分别低于模型组,差异有统计学意义(P＜0.05).高中低剂量组乳酸脱氢酶、磷酸肌酸激酶、超氧化物歧化酶活性及丙二醛含量分别和模型组比较,差异有统计学意义(P＜0.05).结论 辣木籽对大鼠心肌缺血再灌注损伤有保护作用,可能与其抗氧化损伤作用有关.