链球菌和放线菌的胞外代谢物代谢组学鉴定

摘要

目的 检测不同口腔细菌的细胞外代谢产物，发掘各种细菌的特征性代谢谱图，探讨代谢组学在口腔细菌鉴定中的作用。方法取3组细菌生长对数后期培养液，行磁共振氢谱仪检测和主成分分析(PCA)及最小偏二乘法判别分析(PLS-DA)，鉴别各个细菌间的代谢产物。结果3组细菌的代谢产物明显不同，各自聚集、分布区域明显分开；PLS-DA区分效果好于PCA。结论细菌细胞外代谢产物的代谢组学研究，有望成为口腔细菌鉴定和分析的新技术。%Objective To investigate the application of metabonomics in research of oral bacteria, through detecting different of the extracellular metabolites, and find the characteristic metabolic profile of each oral bacteria. Methods 'H-nuclear magnetic resonance (NMR) spectra were obtained from each microbial culture at late exponential growth phase. The obtained data were processed by both the unsupervised principal component analysis (PCA) and the supervised partial least squares discriminant analysis (PLS-DA) to find out the differences among the three groups. Results These 1H-NMR spectrograms of each bacteria are obviously different. The result of pattern recongnition presented marked clustering between the three group. PLS-DA analysis has the better effect to different these samples. Conclusion The results indicated that the 1H-NMR-based extracellular metabonomic approach is a feasible and efficient method for differentiating and analyzing oral bacteria.