复发性阿弗他溃疡患者外周血T-bet、GATA-3和Foxp3的表达及意义

摘要

目的探讨T-bet、GATA-3和Foxp3在复发性阿弗他溃疡（RAU）发病机制中的作用及意义。方法选择RAU患者33例及健康对照30例，采用逆转录聚合酶链反应技术检测患者溃疡发作期外周血单个核细胞（PBMC）中T-bet、GATA-3和Foxp3 mRNA的表达水平，并分析其与RAU免疫功能之间的关系。结果 RAU患者的CD4、CD4/8、T+B+NK细胞含量低于正常值（P<0.01），免疫球蛋白（Ig）A低于正常值（P<0.05），而IgM、血清总补体活性高于正常值（P<0.05）。RAU患者PBMC中T-bet mRNA、T-bet/GATA-3比值、Foxp3 mRNA低于对照组（P<0.01），GATA-3 mRNA高于对照组（P<0.05）；Foxp3表达与T-bet/GATA-3比值之间存在正相关关系（r=0.404，P=0.02）。结论 RAU患者大多存在细胞免疫降低和体液免疫紊乱的问题；其溃疡发作期PBMC中GATA-3表达上升，T-bet表达下降，促进了辅助性T细胞（Th）2优势应答，可能是导致RAU患者免疫功能紊乱的原因之一；而调节性T细胞的数量减少及功能低下，这可能与RAU患者Th1/Th2细胞失衡有关。%Objective??To?elucidate?the?underlying?role?of?T-bet,?GATA-3,?and?Foxp3?in?the?immune?etiology?of?human?with?recurrent?aphthous?ulcer(RAU).?Methods???Peripheral?blood?mononuclear?cells(PBMCs)?were?isolated?by?density?gradient?centrifugation?from?33?cases?of?RAU?and?30?cases?of?healthy?controls.?The?expression?levels?of?T-bet,?GATA-3,?and?Foxp3?mRNA?in?PBMCs?were?investigated?by?reverse?transcription-polymerase?chain?reaction.?The?cellular?and?humoral?immune?status?of?patients?with?RAU?was?investigated?by?flow?cytometry?and?enzyme-linked?immunosorbent?assay.?Results??CD4,?CD4/CD8,?T+B+NK?cells?were?significantly?lower?in?the?RAU?group?than?in?the?control?group(P<0.01).?Immunoglobulin(Ig)?A?was?lower?in?the?RAU?group?than?in?the?control?group(P<0.05).?IgM?and?50%?hemolytic?complement?were?significantly?higher?in?the?RAU?group?than?in?the?control?group(P<0.05).?The?expression?level?of?T-bet?mRNA?and?Foxp3?mRNA,?the?ratio?of?T-bet/GATA-3?in?the?RAU?group?were?lower?than?that?in?the?control?group(P<0.01).?Meanwhile,?the?expression?level?of?GATA-3?mRNA?in?the?RAU?group?was?higher?than?that?in?the?control?group(P<0.05).?A?positive?correlation?was?detected?between?Foxp3?mRNA?level?and?T-bet/GATA-3?ratio(r=0.404,?P=0.02).?Conclusion?Most?RAU?patients?have?immune?cells?reduced?and?humoral?immune?disorder.?The?increased?expression?of?GATA-3?and?decreased?expression?of?T-bet?might?be?one?of?the?mechanisms?of?T?helper?cell(Th)1/Th2?imbalance?in?RAU,?accounting?for?the?abnormal?immune?function?in?patients?with?RAU.?The?decrease?in?regulatory?T?cells?might?be?a?contributing?factor?toward?Th1/Th2?imbalance?in?RAU.