Objective The expression and correlation between cannabinoid receptor type 2 ( CB2R) and autophagy-related protein microtubule-associated protein 1 light chain 3 (LC3) in the hippocampal CA1 re-gion of developing rats with status epilepticus ( SE) were investigated. Methods SE model was established u-sing lithium chloride-pilocarpine intraperitoneally in Sprague-Dawley ( SD) rats and all the rats were randomly divided into four groups ( control group and 3h, 24h, 3d groups after SE). The expressions of CB2R and LC3 in the hippocampal CA1 region at different times were observed using double-label immunofluorescence and Western blotting. Spearman correlational analysis was used to compare the relationship between the two factors. Results Immunofluorescence showed that the expression of CB2R was up-regulated dynamically and peaked at 24h and presented parabolic changes over time. The expression of LC3 changed in accordance with CB2R and e-ven co-expressed with CB2R partly, especially on neurons. Western blotting results furtherly showed the simi-larity of the expression of CB2R and LC3, and finally Spearman correlational analysis presented the significant correlation between these two factors (r=0. 7161, P<0. 05). Conclusion Significant correlation exists be-tween the expression of CB2R and LC3 in the hippocampal CA1 neurons of developing rats with SE, indicating the essential role of CB2R in autophagy regulation of hippocampal CA1 neurons.%目的 观察大鼠癫痫持续状态(status epilepticus,SE)模型中海马CA1区2型大麻素受体(cannabinoid receptor type 2,CB2R)和自噬相关蛋白微管相关蛋白1轻链3(microtubule-associated protein 1 light chain 3,LC3)的表达,并探讨两者的相关性.方法 使用SD大鼠腹腔注射氯化锂-匹鲁卡品建立SE模型,并随机分为4组(即对照组及造模后3 h、24 h、3 d组),应用双重免疫荧光和蛋白印迹法观察SE后不同时间点大鼠海马CA1区CB2R和LC3的表达,后使用Spearman相关性分析比较两者的相关性.结果 免疫荧光结果发现CB2R在SE后呈现动态高表达,并于SE后24 h达到高峰,整体随时间呈现抛物线状变化,而LC3与CB2R变化趋势基本相同,且存在共定位表达,主要表达在神经元中;蛋白印迹实验结果进一步验证了CB2R和LC3的相似表达趋势,Spearman相关性分析发现两者表达趋势具有显著相关性(r=0.7161,P<0.05).结论 SE后大鼠海马CA1区神经元CB2R和LC3的表达具有显著相关性,CB2R可能参与调节SE后海马神经元的自噬过程.
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