Objective To explore the indexes of naturally expectorated sputum cultured by the “semi quantitative 12 order method” to screening the infection of klebsiella pneumoniae(Kp). Methods Smear and inoculation test were performed for each sputum sample at the same time. The quality of samples were confirmed by smear test,and the bacteria phagocytized by white blood cells or the dominant bacteria in the white blood cells centralized region were isolated. The inoculated sputum samples were cultured by ″semi quantitative 12 order method″. The case history of patients, sputum samples of whom were detected with Kp from June 1,2005 to May 31,2010. Then the relationship of the bacteria quantity in the sputum samples at the first time of culture,the quality of samples and the diagnosis of patients. Results The bacteria phagocytized by white blood cells were detected, but the positive rate was not high. There were total 131 strains of Kp. The positive rate of relative number of 0. 1 and ≥ 0. 2 were 52. 78％ and 66.31 ％, at the first time of culture and there was no significant difference between them(X2= 5.24,0.05 ＜P＜0.1). The positive rate of samples at grade A/B and C were 64.77％ and 58.14％ respectively,and there was no significant difference between them (x2= 0.54, P＞0.25). Conclusion In the qualified naturally expectorated sputum samples, if Kp, which cultured by ″semi quantitative 12 order method″ ,is according to the smear,and its quantilty is more than the average carrying quantity in pars oralis pharyngis,susceptibility test should be performed.%目的 探讨自然咳出痰标本半定量培养12级法筛检肺炎克雷伯菌的指标.方法 每份痰标本均同时涂片和接种.涂片观察标本质量,以白细胞吞噬菌或白细胞浓集区域优势菌为拟分离菌;培养采用半定量12级法.查阅2005年6月1日至2010年5月31日住院患者痰内筛检出肺炎克雷伯菌者病历,统计分析首次培养的细菌数量、标本质量与出院诊断的关系.结果 白细胞吞噬菌的确存在,但阳性率不高;肺炎克雷伯菌131株,其首次培养的相对数量为 0.1、≥0.2的阳性率分别为52.78%、66.31%;两者确诊率比较,χ2=5.24,0.05 展开▼
