可溶性髓系细胞触发受体-1在细菌性脑膜炎诊断中的意义

摘要

Objective To investigate the value of soluble triggering receptor expressed on myeloid cells-1(sTRFM-1) in the diagnosis of bacterial meningitis. Methods The levels of sTREM-1 in cerebrospinal fluid ( CSF) were determined by quantitative FlISA assay. The serum levels of PCT and CRP were measured by using immunolumtnometrte assay and immunonephelometry method respectively. The diagnostic value of sTRFM-1 was assessed by receiver operating characteristic(ROC) curve analysis. Results The levels of CSF sTRFM-1 was significantly higher in bacterial meningitis group than in viral meningitis group and control group. There was no obvious difference in CSF sTRFM-1 between viral meningitis group and control group. According to ROC curve, when the cutoff value of CSF sTRFM-1 was set as 25 ng/L,the sensitivity and specificity of it in the diagnosis of bacterial meningitis were 90. 0％ and 93. 5％ ,accuracy was 93. 8 ％ . Conclusion Detection of CSF sTRFM-1 would have certain diagnostic value of bacterial meningitis.%目的 探讨脑脊液中可溶性髓系细胞触发受体-1(sTREM-1)在细菌性脑膜炎中的诊断意义.方法 应用定量酶联免疫吸附法(ELISA)检测脑脊液sTREM-1水平,应用免疫发光法和免疫浊度法分别检测血液中降钙素原(PCT)、C反应蛋白(CRP)水平.应用受试者工作特征 ROC 曲线研究sTREM-1的诊断效能.结果 细菌性脑膜炎组脑脊液sTREM-1 水平较病毒性脑膜炎组和对照组显著升高(P<0.05),病毒性脑膜炎组和对照组比较差异无统计学意义(P>0.05).根据 ROC曲线,取sTREM-1>25 ng/L 为临界值,其曲线下面积为 0.930,诊断细菌性脑膜炎的灵敏度为 90.0%、特异度为93.5%、准确率为93.8%,诊断效能好.结论 测定脑脊液 sTREM-1 水平对于细菌性脑膜炎的诊断有一定价值.