糖皮质激素对类风湿关节炎患者血清中B、T淋巴细胞弱化因子表达的影响

摘要

Objective To investigate the BTLA in the serum level of patients with rheumatoid arthritis in the pathogenesis of the role and regulation of glucocorticoids.Methods ELISA was used to detect the serum levels of BTLA.Western blotting with anti-nuclear antibody ( ANA) spectroscopy, enzyme-linked immunosorbent assay (ELISA) detection of anti-cyclic citrullinated peptide (CCP)antibodies,detected by turbidimetry of rheumatoid factor(RF).Results In the control group, effective cases in 9 cases, 11 cases ineffective;effective cases in the observation group of 15 patients,12 cases ineffective.The clinical observation of 75％ of the total effective rate was higher than 53％(P＜0.05).The control group before treatment serum BTLA(89 ± 13) μg/L, after treatment, slightly elevated serum BTLA(110± ll)μg/L(P＜0.05) ;the observation group before treatment serum BTLA(78 ± 11)μg/ L,the serum of BTLA(140±13)μg/L significantly increased.Conclusion BTLA in rheumatoid arthritis patients with lower levels of serum,glucocorticoids can upregulate the expression,BTLA increase of the disease can affect the outcome of rheumatoid arthritis, can be used as a new therapeutic target.%目的 探讨B、T淋巴细胞弱化因子(BTLA)在类风湿关节炎患者血清中的水平及其在发病机制中的作用和糖皮质激素对其表达的调节.方法 酶联免疫吸附试验(ELISA)法检测患者血清中BTLA的水平.采用免疫印迹法检测抗核抗体(ANA)谱,ELISA法检测抗环瓜氨酸肽(CCP)抗体,免疫比浊法检测类风湿因子(RF).结果 对照组治疗有效例数为9例,无效为11例;观察组有效例数为15例,无效为12例.观察组临床治疗总有效率(75%)明显高于对照组(53%),差异有统计学意义(P<0.05).对照组治疗前血清中BTLA为(89±13)μg/L,治疗后为稍微升高,为(110±11)μg/L,差异有统计学意义(P<0.05);而观察组治疗前血清中BTLA为(78±11)μg/L,治疗后为(140±13)μg/L,明显升高.结论 BTLA在类风湿关节炎患者的血清中水平降低,糖皮质激素可上调其表达,BTLA的升高影响类风湿关节炎病情的转归,可作为治疗的新靶点.