百日咳鲍特菌基因组多态性分析

摘要

Objective To investigate the genomic characterizations of B .pertussis isolates .Methods Genomic polymorphisms of recent B .pertussis isolates were determined by two international universal molecular typing methods,allelic analysis and ML V A for B .pertussis .The diversity indexs of typing methods were analyzed statistically .Results The sequencing analysis of pertacitn(Prn), trachea! colonization factor(tcfA) and pertussis toxin promoter region(ptxP) showed that three allelic profiles Prnl/ptxPl/tcfA2, Prn2/PtxP3/tcfA2 and Prn3/ptxPl/tcf A2 wore; found with the; frequency of 71 .43%,19 .05% and 9 .52% in the; strains analyzed, respectively .21 isolates produced 12 different B .pertussis MLV A profiles,and a new profile was found .Main ML vA profiles were MLVA-136 and MLVA-152,accounted 28 .57% and for 19 .05% respectively .The results of statistical analysis suggested that ML-VA were better than allelic analysis for molecular typing of B .pertussis .Conclusion These results demonstrated that B .pertussis epidemic strains in some regions of China were different from those in European countries .These works could allow us to gain more insights into the molecular epidemiological patterns of the B .pertussis strains facilitating for a better control of pertussis spread and new vaccine prevention strategies in China .%目的 了解百日咳鲍特菌(简称百日咳杆菌)基因组特征.方法 应用百日咳杆菌等位基因分型法和多位点可变数量串联重复序列分析(MLVA)法分析百日咳杆菌基因多态性,同时对2种分型方法的多态性指数进行统计学分析.结果 百日咳黏附素(Prn)、支气管黏附因子(tcfA)和百日咳毒素基因上游启动子区域(ptxP)3种等位基因序列分析结果显示,共发现3种等位基因组合型:Prn1/ptxP1/tcfA2、Prn2/ptxP3/tcfA2和Prn3/ptxP1/tcfA2,所占比例分别为71.43%、19.05%和9.52%.MLVA法将21株分离菌株分成了12个不同的MLVA型,MLVA-136型和MLVA-152型为主要流行MLVA型,所占比例分别为28.57%和19.05%,并发现1种新MLVA型菌株.统计学分析表明MLVA法较等位基因分型法具有更高的分辨率.结论 国内部分地区百日咳杆菌流行菌株与欧洲其他国家不同,掌握百日咳杆菌分子流行病学的第一手资料,对有效预防和控制百日咳的流行以及制定新的百日咳疫苗免疫策略具有指导意义.